Analysis of Microbial Community Structure of Channel Catfish Ictalurus punctatus Intestine and Culture Water
XIONG Xiangying1, ZHAO Yanfei1,2, WANG Zhicheng1
1.Guangxi Institute of Oceanology Limited Liability Company, Beihai 536000, China; 2.Laboratory of Crustacean Genetics, Breeding and Reproduction, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China
Abstract：In order to study the structure of intestinal microflora of healthy channel catfish and its relationship with pond culture water, the composition and diversity of microbial communities were comparatively investigated in the intestinal tract and culture water by 16S rDNA high-throughput sequencing and bioinformatics analysis method. The results showed that the abundance and diversity of intestinal flora of channel catfish were lower than those of culture water through diversity index analysis. 69.57% of OTUs in channel catfish was consistent with that in water. However, the dominant microorganisms in the water were Proteobacteria, Actinobacteria and Cyanobacteria, while the dominant microfloras in the intestinal tract were Bacteroidetes, Fusobacteria and Firmicutes. There was no significant correlation between the composition and abundance of the dominant microflora in the intestinal tract and the culture water. The composition of the intestinal microflora had certain independence, and its dominant bacterial species were almost independent of the aquaculture environment. PICRUSt prediction of the bacterial phenotype revealed that anaerobes and Gram-positive bacteria were the main bacteria in the intestinal tract of channel catfish. The biofilm formation and pathogenicity potential phenotype showed that there were a large number of opportunistic pathogens in the intestinal tract and water. In this study, the average relative abundances of Cetobacterium and Bacteroides in intestinal flora ofchannel catfish were 36.60% and 15.82%, indicating that the dominant probiotics in intestinal microorganism of channel catfish were mainly Cetobacterium and Bacteroides. The finding provides theoretical basis for healthy breeding of channel catfish and screening of probiotics.
[1]HAWKE J P, MCWHORTER A C, STEIGERWALT A G, et al. Edwardsiella ictaluri sp. nov., the causative agent of enteric septicemia of catfish[J].International Journal of Systematic Bacteriology,1981,31(4):396-400. [2]ARIAS C R, CAI W L, PEATMAN E, et al. Catfish hybrid Ictalurus punctatus×I.furcatus exhibits higher resistance to columnaris disease than the parental species[J].Diseases of Aquatic Organisms,2012,100(1):77-81. [3]余晓丽,陈明,李超,等.斑点叉尾鮰暴发性海豚链球菌病的研究[J].大连水产学院学报,2008,23(3):185-191. [4]刘堂水,汪成竹,陈昌福.斑点叉尾鮰细菌性病原的分离与鉴定[J].华中农业大学学报,2006,25(5):550-554. [5]FLINT H J, BAYER E A, RINCON M T, et al. Polysaccharide utilization by gut bacteria:potential for new insights from genomic analysis[J].Nature Reviews Microbiology,2008,6(2):121-131. [6]VILLAMIL L, REYES C, MARTÍNEZ-SILVA M A.In vivo and in vitro assessment of Lactobacillus acidophilus as probiotic for tilapia (Oreochromis niloticus, Perciformes:Cichlidae) culture improvement[J].Aquaculture Research,2014,45(7):1116-1125. [7]薛明,何瑶瑶,邱孟德,等.高通量测序分析凡纳滨对虾育苗期水体菌群结构特征[J].水产学报,2017,41(5):785-794. [8]BLANCHETON J P, ATTRAMADAL K J K, MICHAUD L, et al. Insight into bacterial population in aquaculture systems and its implication[J].Aquacultural Engineering,2013,53:30-39. [9]李学梅,余育和,解绶启,等.三种室内饲养鱼类肠道微生物群落PCR-DGGE指纹分析[J].水生生物学报,2011,35(3):423-429. [10]MICHELSEN C F, PEDAS P, GLARING M A, et al. Bacterial diversity in Greenlandic soils as affected by potato cropping and inorganic versus organic fertilization[J].Polar Biology,2014,37(1):61-71. [11]李革雷,陈昌福,高宇,等.3种养殖模式水体中细菌多样性研究[J].华中农业大学学报,2012,31(3):381-390. [12]吴欢欢,王伟继,吕丁,等.应用高通量测序技术分析大菱鲆幼鱼肠道及其养殖环境的微生物群落结构[J].渔业科学进展,2019,40(4):84-94. [13]张正,李彬,王印庚,等.基于高通量测序的池塘养殖半滑舌鳎消化道菌群的结构特征分析[J].水生生物学报,2015,39(1):38-45. [14]王贤丰,赵艳飞,宋志飞,等.应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构[J].中国水产科学,2017,24(6):1245-1253. [15]孙振丽,宣引明,张皓,等.南美白对虾养殖环境及其肠道细菌多样性分析[J].中国水产科学,2016,23(3):594-605. [16]张琛,王岩,郑侠飞,等.海水网箱养殖花鲈和日本黄姑鱼肠道细菌的多样性[J].中国水产科学,2020,27(9):1113-1124. [17]PAERL H W, DYBLE J, MOISANDER P H, et al. Microbial indicators of aquatic ecosystem change:current applications to eutrophication studies[J].FEMS Microbiology Ecology,2003,46(3):233-246. [18]罗鹏,胡超群,谢珍玉,等.凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析[J].热带海洋学报,2006,25(2):49-53. [19]ZHANG C, ZHENG X F, REN X, et al. Bacterial diversity in gut of large yellow croaker Larimichthys crocea and black sea bream Sparus macrocephalus reared in an inshore net pen[J].Fisheries Science,2019,85(6):1027-1036. [20]CHAIYAPECHARA S, RUNGRASSAMEE W, SURIYACHAY I, et al. Bacterial community associated with the intestinal tract of P.monodon in commercial farms[J].Microbial Ecology,2012,63(4):938-953. [21]VAN KESSEL M A, DUTILH B E, NEVELING K, et al. Pyrosequencing of 16S rRNA gene amplicons to study the microbiota in the gastrointestinal tract of carp (Cyprinus carpio L.)[J].AMB Express,2011,1:41. [22]DI MAIUTA N, SCHWARZENTRUBER P, SCHEN-KER M, et al. Microbial population dynamics in the faeces of wood-eating loricariid catfishes[J].Letters in Applied Microbiology,2013,56(6):401-407. [23]BORSODI A K, SZABÓ A, KRETT G, et al. Gut content microbiota of introduced bigheaded carps (Hypophthalmichthys spp.) inhabiting the largest shallow lake in Central Europe[J].Microbiological Research,2017,195:40-50. [24]LI X M, ZHU Y J, YAN Q Y, et al. Do the intestinal microbiotas differ between paddlefish (Polyodonspatha-la) and bighead carp (Aristichthys nobilis) reared in the same pond?[J].Journal of Applied Microbiology,2014,117(5):1245-1252. [25]李建柱,侯杰,张鹏飞,等.鱼菜共生模式中不同鱼类肠道微生物群落结构的比较[J].南方水产科学,2016,12(6):42-50. [26]LARSEN A M, MOHAMMED H H, ARIAS C R. Characterization of the gut microbiota of three commercially valuable warmwater fish species[J].Journal of Applied Microbiology,2014,116(6):1396-1404. [27]BLEDSOE J W, WALDBIESER G C, SWANSON K S, et al. Comparison of channel catfish and blue catfish gut microbiota assemblages shows minimal effects of host genetics on microbial structure and inferred function[J].Frontiers in Microbiology,2018,9:1073. [28]TSUCHIYA C, SAKATA T, SUGITA H. Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish[J].Letters in Applied Microbiology,2008,46(1):43-48. [29]ROESELERS G, MITTGE E K, STEPHENS W Z, et al. Evidence for a core gut microbiota in the zebrafish[J].The ISME Journal,2011,5(10):1595-1608. [30]BÄCKHED F, DING H, WANG T, et al. The gut microbiota as an environmental factor that regulates fat storage[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(44):15718-15723. [31]SEARS C L. A dynamic partnership: celebrating our gut flora[J].Anaerobe,2005,11(5):247-251. [32]SAHA S, ROY R N, SEN S K, et al. Characterization of cellulase-producing bacteria from the digestive tract of tilapia, Oreochromismossambica (Peters) and grass carp, Ctenopharyngodon idella (Valenciennes) [J].Aquaculture Research,2006,37(4):380-388. [33]蒋长苗,鲍传和,马元山.草鱼肠道正常菌群与肠炎病原菌关系的初步研究[J].吉林农业大学学报,1992,14(1):55-58. [34]SAKATA T, KAKIMOTO D, SUGITA H, et al. Characteristics of obligate anaerobic bacteria in the intestines of freshwater fish[J]. Bulletin of the Japanese Society of Scientific Fisheries,1981,47(3):421-427. [35]侯进慧,陈宏伟,曹泽虹,等.鲫鱼肠道细菌菌群初步分析[J].食品科学,2010,31(11):178-181. [36]GUPTA P, SARKAR S, DAS B, et al. Biofilm, pathogenesis and prevention— a journey to break the wall: a review[J].Archives of Microbiology,2016,198(1):1-15.
2022, Vol. 41 
