Effects of Different Environmental Factors on Infection of Enterocytozoon hepatopenaei in Ridgetail White Prawn Exopalaemon carinicauda
DUAN Jiancheng1, HU Jihui1, MU Jinxin1, DENG Gaowei1, GAO Wei1, MU Hua1,2,3, ZHANG Qingqi4, GAO Huan1,2,3
1. Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University,Lianyungang 222005, China; 2. Jiangsu Marine Biological Industry Technology Collaborative Innovation Center, Lianyungang,222005, China; 3. The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing210014, China; 4. Lianyungang Ganyu Jiaxin Aquatic Products Development Co., Ltd., Lianyungang 222100, China
Abstract：The spread of Enterocytozoon hepatopenaei (EHP) is related to the host physique and water environment. Environmental factors such as temperature, salinity, and ammonia nitrogen concentration are extremely important influencing factors in the breeding process. It is shown that too high or too low temperature and salinity have a greater impact on the specific growth rate and non-specific immunity of ridgetail prawn Exopalaemon carinicauda. In this study, the EHP capacity in ridgetail prawn was used as an indicator to determine the transmission rate of EHP. The interaction of temperature, salinity and ammonia nitrogen on the transmission of EHP were discussed. In this experiment, three factors and three levels of orthogonal culture experiment were designed, including temperature (22 ℃, 24 ℃, 26 ℃), salinity (20, 24, 28) and ammonia nitrogen concentration (2 mg/L, 4 mg/L, 6 mg/L). Through a ten-day artificial infection experiment, real-time fluorescence quantitative PCR was used to detect EHP load under different environmental factors. The results showed that under the experimental conditions, the EHP capacity increased significantly (P<0.05). The influence of environmental factors on EHP transmission in the shrimp was temperature, ammonia nitrogen and salinity. The optimum propagation conditions were temperature 24 ℃, ammonia nitrogen 2 mg/L and salinity 28, and the unsuitable conditions were temperature 26 ℃, ammonia nitrogen 4 mg/L and salinity 20. The findings ascertained the environmental conditions for the transmission of intestinal worms in shrimp, and provided data reference for the prevention and control of intestinal cysts in shrimp in marine aquaculture.
段健诚, 胡吉卉, 穆金鑫, 邓高威, 高威, 牟华, 张庆起, 高焕. 不同环境因素对虾肝肠胞虫感染脊尾白虾的影响研究[J]. 水产科学, 2023, 42(2): 296-302.
DUAN Jiancheng, HU Jihui, MU Jinxin, DENG Gaowei, GAO Wei, MU Hua, ZHANG Qingqi, GAO Huan. Effects of Different Environmental Factors on Infection of Enterocytozoon hepatopenaei in Ridgetail White Prawn Exopalaemon carinicauda. Fisheries Science, 2023, 42(2): 296-302.
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2023, Vol. 42 
