Effects of Different Diets on Metamorphosis of Megalopa in Mud Crab Scylla paramamosain
QIAO Guangde1,2, MA Chunyan2, ZHANG Fengying2, WANG Wei2, ZHAO Ming2, CHEN Wei2, FU Yin1,2 , LIU Zhiqiang2, MA Lingbo2
1. College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; 2. Genetic Breeding and Biotechnology Laboratory, East China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China
Abstract：In order to find the suitable diets for megalopa of mud crab Scylla paramamosain during its metamorphosis period, (1000±30) megalopa larvae were reared in an incubation bucket containing 360 L water and fed Artemia nauplius, frozen copepods, frozen adult Artemia, formulated feed and no feeding until the metamorphosed into crabs to evaluate effects of feeding different diets on the water quality in aquaculture water, the molting metamorphosis of the larvae, the activity of immune-related enzymes and digestive enzymes. The results showed that there were the minimal ammonia nitrogen content and nitrite content and the maximal survival rate in the cultured water in Artemia nauplius group. The megalopa larvae had 1—3 days shorter development period in the Artemia nauplii group than that in the other three groups, with significantly longer carapace length and carapace width than those in the formulated diets group, without significant difference between frozen copepods group and frozen Artemia groups. There were no significant differences in acid phosphatase activity, alkaline phosphatase activity, superoxide dismutase activity and α-amylase activity among the four groups. The lysozyme activity and total antioxidant capacity were shown to be not significantly different in the Artemia nauplius group from those in frozen copepods and frozen Artemia groups, significantly higher than those in the formulated diet group. There were significantly higher trypsin and lipase activities in Artemia group than those in the other three groups, indicating that the Artemia nauplii were more suitable as a diet for the megalopa of mud crab, which positively affected the water quality, the molting survival rate, immunoenzyme activity and digestive activity of the juvenile crab. The effects of frozen copepods and frozen Artemia were both better than formulated diets, though they had slightly worse effect. It was found that frozen copepods and frozen Artemia were used as partial substitutes for Artemia nauplii during the larval rearing of the mud crab megalopa, which can help to reduce the production costs, simplify the production process, and eventually promote the large-scale production of seedlings in this crab. The findings provided the basis for the selection of food during the artificial cultivation of the mud crab seed.
[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2021中国渔业统计年鉴[M].北京:中国农业出版社,2021. [2] 王桂忠,李少菁,陈志刚.青蟹(Scylla spp.)养殖现状及拟穴青蟹(S. paramamosain)种群生物学研究[J].厦门大学学报(自然科学版),2016,55(5):617-623. [3] 顾孝连,乔振国.我国蟹类土池育苗技术研究进展[J].海洋渔业,2012,34(1):110-116. [4] 乔振国,高露娇.海水蟹类安全生产技术指南[M].北京:中国农业出版社,2012. [5] 杨志刚,刘启彬,姚琴琴,等.养殖密度和饵料组成对河蟹仔蟹生长和存活的影响[J].生物学杂志,2015,32(6):34-39. [6] 姚高友,吴浩敏,谭杰,等.不同人工饵料对方斑东风螺幼虫生长及存活的影响[J].水产科学,2018,37(1):115-119. [7] 陈建华,张庆起,高焕,等.不同饵料搭配对脊尾白虾幼体发育的影响[J].水产科学,2017,36(5):662-665. [8] 陈学雷,李少菁,王桂忠,等.拟穴青蟹大眼幼体相残与资源可获得性和饥饿关系的研究[J].厦门大学学报(自然科学版),2008,47(1):99-103. [9] HOLME M H, ZENG C S, SOUTHGATE P C. A review of recent progress toward development of a formulated microbound diet for mud crab, Scylla serrata, larvae and their nutritional requirements[J]. Aquaculture,2009,286(3/4):164-175. [10] SHAO L C, WANG C, HE J, et al. Meat quality of Chinese mitten crabs fattened with natural and formulated diets[J]. Journal of Aquatic Food Product Technology,2014,23(1):59-72. [11] 苏贵森,肖国强,蔡景波,等.不同饵料对拟穴青蟹生长、酶活性及生化指标的影响[J].海洋湖沼通报,2022,44(2):41-48. [12] WAIHO K, FAZHAN H, QUINITIO E T, et al. Larval rearing of mud crab (Scylla):what lies ahead[J]. Aquaculture,2018,493:37-50. [13] 成永旭,王武,吴嘉敏,等.虾蟹类幼体的脂类需求及脂类与发育的关系[J].中国水产科学,2000,7(4):104-107. [14] 艾春香,林琼武,李少菁,等.蟹类的营养需求研究及其配合饲料研制[J].厦门大学学报(自然科学版),2006,45(增刊2):205-212. [15] 艾春香,刘建国,林琼武,等.青蟹的营养需求研究及其配合饲料研制[J].水产学报,2007,31(增刊):122-128. [16] 蔡恒辉,曹琛,邢佐平,等.不同饵料对中华虎头蟹幼体发育和存活率的影响[J].水产养殖,2014,35(4):11-15. [17] 肖起珍,杜雪,刘青,等.不同活饵料对中华绒螯蟹大眼幼体生长发育的影响[J].生物学杂志,2017,34(4):33-37. [18] 施永海,张根玉,张海明,等.配合饲料和活饵料对刀鲚幼鱼生长、存活和消化酶、非特异性免疫酶、代谢酶及抗氧化酶活性的影响[J].水产学报,2014,38(12):2029-2038. [19] 李柄男.凡纳滨对虾生物饵料育苗工艺优化与中间暂养问题的初步研究[D].青岛:中国海洋大学,2015. [20] 沈桂宇.主要污染因子对虾蟹健康影响的研究进展[J].饲料工业,2013,34(22):47-51. [21] 刘国锋,徐跑,吴霆,等.中国水产养殖环境氮磷污染现状及未来发展思路[J].江苏农业学报,2018,34(1):225-233. [22] 刘凯,方涛,冯志华,等.藻类处理水产养殖废水的研究进展[J].淮海工学院学报(自然科学版),2016,25(1):74-79. [23] 蔡逸龙,张利兵,胡高宇,等.不同饵料对室内立体养殖拟穴青蟹生长与摄食环境的影响[J].浙江农业学报,2022,34(11):2404-2415. [24] VAZQUEZ L, ALPUCHE J, MALDONADO G, et al. Review:immunity mechanisms in crustaceans[J]. Innate Immunity,2009,15(3):179-188. [25] 李晨露,李京昊,成永旭,等.不同饵料对克氏原螯虾仔虾养殖水质、生长、消化酶和非特异性免疫酶的影响[J].上海海洋大学学报,2022,31(4):883-892. [26] 周景,黄亚冬,周旋,等.两种饵料和投喂频率对三疣梭子蟹生长、消化和免疫指标的影响[J].天津农学院学报,2021,28(4):41-46. [27] 何亮银,史晓丽,周逢芳,等.低盐胁迫对大黄鱼非特异性免疫酶活力的影响[J].应用海洋学学报,2022,41(2):347-354. [28] HIKIMA S, HIKIMA J I, ROJTINNAKORN J, et al. Characterization and function of kuruma shrimp lysozyme possessing lytic activity against Vibrio species[J]. Gene,2003,316:187-195. [29] 何海琪,孙凤.中国对虾酸性和碱性磷酸酶的特性研究[J].海洋与湖沼,1992,23(5):555-560. [30] 李世顺,乔雁冰,左然涛,等.不同饵料对海蜇幼体生长、消化酶、抗氧化和免疫酶活力的影响[J].中国渔业质量与标准,2021,11(2):32-37. [31] 唐媛媛.饲料蛋白质水平对卵形鲳鲹消化酶、非特异性免疫和肠道内容物细菌的影响[D].厦门:厦门大学,2014. [32] 刘立鹤,陈立侨,周永奎,等.甲壳动物消化酶的研究[J].饲料工业,2006,27(18):56-62. [33] 董兰芳,童潼,张琴,等.饲料碳水化合物水平对拟穴青蟹稚蟹生长、体成分和消化酶活性的影响[J].水生生物学报,2019,43(2):252-258. [34] 董兰芳,张琴,许明珠,等.饲料糖脂比对拟穴青蟹仔蟹生长性能、体组成和消化酶活性的影响[J].动物营养学报,2017,29(3):979-986. [35] 江洪波,陈立侨,王群,等.饵料蛋白质对中华绒螯蟹仔蟹消化酶活性及胰蛋白酶mRNA丰度的影响[J].水产学报,2005,29(2):216-221. [36] PEOPLE LE RUYET J, ALEXANDRE J C, THÉBAUD L, et al. Marine fish larvae feeding:formulated diets or live prey?[J]. Journal of the World Aquaculture Society,1993,24(2):211-224. [37] JONES D A, KUMLU M, LE VAY L, et al. The digestive physiology of herbivorous, omnivorous and carnivorous crustacean larvae:a review[J]. Aquaculture,1997,155(1/2/3/4):285-295. [38] SHIAU S Y, PENG C Y. Utilization of different carbohydrates at different dietary protein levels in grass prawn, Penaeus monodon, reared in seawater[J]. Aquaculture,1992,101(3/4):241-250. [39] 王林华,沈南南,江海仪,等.不同生物饵料组合对脊尾白虾幼体变态发育的影响[J].渔业科学进展,2022,43(4):171-179. [40] 吴雷明,韩光明,寇祥明,等.3种生物饵料对宽体金线蛭幼蛭生长性能、消化酶活性及免疫力的影响[J].动物营养学报,2019,31(7):3383-3390.
2024, Vol. 43 
