Isolation, Identification and Pathological Observation of Ulcerative Disease Syndrome in Xenocypris davidi
WANG Ronghua1, LIU Yisha1, FU Yanchun1, ZHANG Runchun1, ZHOU Yong2, LUO Yushuang1, LIU Fei1, CHEN Zhongyuan1
1. Hunan Provincial Key Laboratory for Molecular Immunity Technology of Aquatic Animal Diseases, Key Laboratory of Health Aquaculture and Product Processing in Dongting Lake Area of Hunan Province, Changde Research Center for Agricultural Biomacromolecule, Hunan University of Arts and Science, Changde 415000, China; 2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China
Abstract：Ulcerative disease syndrome in Xenocypris davidi occurred in a fish farm of Zhuzhou city in Hunan Province during the breeding season for the past few years, causing ulcers in different positions of broodstock fish body surface. To determine the pathogenesis and histopathological changes in the ulcerative disease syndrome of X. davidi and explore the prevention and control measures of this disease, the traditional methods of bacterial isolation and histopathology were used in the research, and the sensibility of the pathogenic bacteria to antibiotics and Chinese herbal medicines were detected by the KB paper method. In this study, the dominant strain HZ0416 was isolated from the liver of the morbid X. davidi with typical symptom of ulcerative disease syndrome and identified as Aeromonas schubertii through 16S rDNA gene sequencing and biological identification. The artificial infection test verified that HZ0416 caused the ulcerative disease syndrome in yellow catfish Pelteobagrus fulvidraco. The drug susceptibility analysis showed that HZ0416 was significantly sensitive to gentamicin, florfenicol and nutgall, and sensitive to 8 drugs such as ciprofloxacin, herba houttuyniae, herba andrographitis, but low sensitive or resistant to 22 drugs such as kanamycin, polymyxin B, Cortex Fraxini, and Folium Artemisiae Argyi. Pathological section showed that the diseased fish suffered from multiple organ tissue hyperemia and hemorrhage, accompanied by inflammatory cell infiltration, substantial cell vacuolization and necrosis. A. schubertii was the potential pathogen of ulcerative disease syndrome in X. davidi, and prevented during the breeding process by feeding florfenicol and Galla Chinensis.
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2021, Vol. 40 
