Screening, Identification and Biological Characteristics of Two Antagonistic Bacteria Against Aquaculture Aeromonas Pathogens
LONG Meng1,2,3, FAN Huimin1, JIANG Yao3, XIA Hongli1, CHENG Jun1, YU Dapeng1, XIA Liqun1,4, LU Yishan1,4
1. Shenzhen Public Service Platform for Evaluation of Marine Economic Animal Seedings, Guangdong Provincial Engineering Research Center for Aquatic Animal Health Assessment, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; 2. Shenzhen Dapeng New District Science and Technology Innovation Service Center, Shenzhen 518120, China; 3. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China; 4. Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China
Abstract：Aeromonas is an important pathogen of aquaculture, and there are still no effective treatments to prevent the infections caused by various Aeromonas. Mangrove sediments possess abundant Bacillus, becoming important reservoirs of bioactive microorganisms. In this study, we isolated two strains from mangrove sediments in Dongchong, Shenzhen, which displayed strong antagonistic activity against six pathogenic Aeromonas species. Based on the analysis of morphological properties, biochemical characteristics and molecular genetics, the two strains named AH10 and AQ1 were identified as B. amyloliquefaciens and B. velezensis respectively. The biological characteristics of the two strains showed similar optimum conditions of 32 ℃, pH of 5—7 and salinity of 0—20 for growth. The results of antibiotics sensitivity test showed that the two strains were highly sensitive to most of the antibiotics, except tetracycline and low dose streptomycin. Besides the six pathogenic Aeromonas, the antagonistic results revealed that the two strains also had antagonistic activities against Edwardsiella, and Plesiomonas shigelloides, in which B. amyloliquefaciens AH10 and B. velezensis AQ1 showed the strongest effect against P. shigelloides and A. salmonicida respectively. The two strains also showed no pathogenic capacity to zebrafish Danio rerio and silver carp Hypophthalmichthys molitrix. In conclusion, new antagonistic resources for the biological control of pathogenic Aeromonas in aquaculture is reported, providing good application prospects and research potential in the future.
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2022, Vol. 41 
