Indoor Biofloc Culture of Pacific White Shrimp Litopenaeus vannamei in Low Temperature Season
ZHOU Hui1, HAN Manqiang1,2, TANG Baogui1, WU Xumin1, DU Shumin1, TAN Ruihua1, GONG Hanfu1, LIN Zhihao1, HE Yixun1, ZHONG Yuqi1, LIN Guiteng1
1. Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal, Regular High Education Institute of Guangdong Province, Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China; 2. Zhanjiang Haisite Aquatic Products Technology Co., Ltd., Zhanjiang 524088, China
Abstract:In low temperature season, the growth index and body composition of Pacific white shrimp Litopenaeus vannamei cultured in the indoor biofloc system were studied. In the experiment, clean water culture (CW, fed ad libitum) was set as the control group, and biofloc culture (BF, 70% feeding amount of the CW group) was set as the experimental group. There were 3 tanks in each group and 18 individuals of Pacific white shrimp (3.17±0.37) g per tank cultured for 42 days, recorded the feeding rate of Pacific white shrimp, measured the body weight and body composition of the Pacific white shrimp, and calculated the growth index. The results showed: (1) the average water temperature in the CW group was (21.3±2.0) ℃, the average daily food intake rate of Pacific white shrimp was (1.87±0.30)%, which did not change significantly (P>0.05), and there was no significant correlation between water temperature and the average daily food intake rate of Pacific white shrimp (P>0.05). (2) From 31 to 42 days, the water temperature of the BF group was significantly higher than that in the CW group (P<0.05). (3) With the food intake rate only 70% in the CW group, the final body weight, weight gain rate, specific growth rate and body composition of Pacific white shrimp in the BF group and the CW group were not significantly different (P>0.05). There were significantly higher feed conversion efficiency and protein efficiency in the Pacific white shrimp in the BF group than those in the CW group (P<0.05). It was found that in the low temperature season, compared with clean water culture, the indoor biofloc culture mode effectively improved the feed conversion ratio of the Pacific white shrimp and maintained higher water temperature, while the growth rate of the Pacific white shrimp did not showed significant difference.
[1] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.2019中国渔业统计年鉴[M].北京:中国农业出版社,2019. [2] 张井增,马建军,孙志新,等.南美白对虾产业发展及瓶颈综述[J].河北渔业,2018(9):48-51. [3] 朱林,车轩,刘兴国,等.对虾工厂化养殖研究进展[J].山西农业科学,2019,47(7):1288-1290. [4] BOYD C E. Pond water aeration systems[J].Aquacultural Engineering,1998,18(1):9-40. [5] AVNIMELECH Y. Carbon/nitrogen ratio as a control element in aquaculture systems[J].Aquaculture,1999,176(3/4):227-235. [6] CRAB R, DEFOIRDT T, BOSSIER P, et al. Biofloc technology in aquaculture:beneficial effects and future challenges[J].Aquaculture,2012,356/357:351-356. [7] BURFORD M A, THOMPSON P J, MCINTOSH R P, et al. The contribution of flocculated material to shrimp (Litopenaeus vannamei) nutrition in a high-intensity, zero-exchange system[J].Aquaculture,2004,232(1/2/3/4):525-537. [8] AVNIMELECH Y, KOCHBA M. Evaluation of nitrogen uptake and excretion by tilapia in biofloc tanks, using 15N tracing[J].Aquaculture,2009,287(1/2):163-168. [9] CARDONA E, LORGEOUX B, GEFFROY C, et al. Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc:assessment by C and N stable isotope ratios and effect on key digestive enzymes[J].Aquaculture,2015,448:288-297. [10] KUHN D D, BOARDMAN G D, LAWRENCE A L, et al. Microbial floc meal as a replacement ingredient for fish meal and soybean protein in shrimp feed[J].Aquaculture,2009,296(1/2):51-57. [11] 孙振.产絮团微生物在凡纳滨对虾养殖中的作用[D].青岛:中国海洋大学,2013. [12] GAMBOA-DELGADO J, RODRíGUEZ MONTES DE OCA G A, ROMÁN REYES J C, et al. Assessment of the relative contribution of dietary nitrogen from fish meal and biofloc meal to the growth of Pacific white shrimp (Litopenaeus vannamei)[J].Aquaculture Research,2017,48(6):2963-2972. [13] 赵大虎.添加不同碳源对生物絮团组分和凡纳滨对虾生理健康、生长的影响[D].青岛:中国海洋大学,2013. [14] KIM S K, PANG Z G, SEO H C, et al. Effect of bioflocs on growth and immune activity of Pacific white shrimp, Litopenaeus vannamei postlarvae[J].Aquaculture Research,2014,45(2):362-371. [15] 张亚卓,郑忠明,华建权,等.低盐度条件下生物絮团的营养组分及凡纳滨对虾和银鲫对其摄食效率的研究[J].宁波大学学报(理工版),2016,29(3):29-33. [16] 张哲,杨章武,葛辉,等.不同碳源对凡纳滨对虾育苗标粗水体生物絮团的结构、营养成分、细菌群落及其水质的影响[J].水产学报,2019,43(3):639-649. [17] 王超,潘鲁青,张开全.生物絮团在凡纳滨对虾零水交换养殖系统中的应用研究[J].海洋湖沼通报,2015(2):81-89. [18] LARA G, HOSTINS B, BEZERRA A, et al. The effects of different feeding rates and re-feeding of Litopenaeus vannamei in a biofloc culture system[J].Aquacultural Engineering,2017,77:20-26. [19] VOL N. Official Methods of Analysis of AOAC International (16th edn)(Patricia A. Cunniff, ed.)[J]. Trends in Food Science and Technology,1995,6(11):382. [20] 陈昌生,黄标,叶兆弘,等.南美白对虾摄食、生长及存活与温度的关系[J].集美大学学报(自然科学版),2001,6(4):296-300. [21] 安文强,黎文伟,谭北平,等.凡纳滨对虾对饲料中钙、磷的营养需求[J].水产科学,2020,39(1):1-11. [22] 朱学芝,郑石轩,潘庆军,等.芽孢杆菌对凡纳滨对虾免疫和生化指标的影响[J].饲料研究,2007(4):56-59. [23] 李卓佳,郭志勋,冯娟,等.应用芽孢杆菌调控虾池微生态的初步研究[J].海洋科学,2006,30(11):28-31. [24] ZIAEI-NEJAD S, REZAEI M H, TAKAMI G A, et al. The effect of Bacillus spp.bacteria used as probiotics on digestive enzyme activity, survival and growth in the Indian white shrimp Fenneropenaeus indicus[J].Aquaculture,2006,252(2/3/4):516-524. [25] 陈伟.功能性生物絮团和BFT系统的构建及其在对虾养殖中的应用效果研究[D].上海:上海海洋大学,2018. [26] 赵先锋.利用农业副产品发酵坚强芽孢杆菌及其用于加强生物絮团培养的研究[D].青岛:中国海洋大学,2011. [27] 田道贺,桂福坤,李华,等.硝化型生物絮团的驯化培养[J].南方水产科学,2019,15(4):39-45. [28] 秦海鹏,杨世平,王博,等.不同盐度对生物絮团、对虾生长以及酶活性的影响[J].水产科学,2020,39(3):400-406. [29] CRAB R, CHIELENS B, WILLE M, et al. The effect of different carbon sources on the nutritional value of bioflocs, a feed for Macrobrachium rosenbergii postlarvae[J].Aquaculture Research,2010,41(4):559-567. [30] 张扬,杨逸尊,陈晓庆,等.不同粒径的生物絮团氨氮处理能力和营养成分组成[J].中国水产科学,2020,27(3):295-306. [31] CHAN-VIVAS E, EDÉN M G, MALDONADO C, et al. Does biofloc improve the energy distribution and final muscle quality of shrimp, Litopenaeus vannamei (Boone, 1883)?[J].Journal of the World Aquaculture Society,2019,50(2):460-468. [32] 葛海伦,朱锦裕,赵臣泽,等.生物絮团对罗氏沼虾体组成和消化酶活性的影响[J].淡水渔业,2017,47(3):66-72. [33] 申玉春,齐明,朱春华,等.凡纳滨对虾不同生长阶段食物组成结构的研究[J].广东海洋大学学报,2010,30(1):44-49.