Cloning and Expression Profile of Clock Gene cDNA in Schizothorax o'connori
ZHOU Jianshe1,2, TASHI Lhamo1, WANG Wanliang1, SUN Shuaijie2, ZHANG Chi1
1. Institute of Fisheries Science,Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850032, China; 2. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450002, China
Abstract：Schizothorax o'connori is widely distributed in the Brahmaputra River and spawns from March to May, showing obvious seasonal breeding behavior. The Clock gene was considered as a potential target gene for natural selection to shape the daily rhythm and to be related to life history characteristics such as animal reproduction. The variation in the length of C-terminal Poly-Q of Clock protein of S. o'connori may be involved in the onset of oviposition. In order to establish the foundation for understanding of the important role of Clock gene of Schizothorax in Qinghai-Xizang Plateau in seasonal breeding behavior, the complete cDNA sequence of Clock gene in S. o'connori were cloned by RT-PCR and RACE-PCR, and the expression profile were detected in liver, spleen, heart, brain, gonad and muscle of both male and female. The results showed that the complete cDNA sequence of Clock gene was 4291 bp (GenBank: MT774361),5'UTR was 539 bp, 3'UTR was 1046 bp, CDS was 2706 bp, and encoded 901 amino acid, with typical Poly-Q structure in the protein sequence. The expression profile of Clock gene was existed in all detected tissue of S. o'connori, with the maximum in gonad and the minimum in spleen. The expression profile of all detected tissue revealed a trend that higher expression profile in female than that in male, except heart. The high expression profile of Clock gene in both female and male gonad illustrated that more active important role in reproduction process of S. o'connori, and the insertion of large fragments of Clock protein may also be specialized to accommodate certain conditions at high altitudes.
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2023, Vol. 42 
