Effect of Alizarin Red S on Marking of Juvenile Schizothorax o′connori
HE Wenjia1, ZENG Benhe1, ZHU Chengke2, ZHOU Chaowei2
1. Institute of Fisheries Science, Xizang Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850002, China; 2. School of Fisheries, Rongchang Campus, Southwest University, Chongqing 400715, China
Abstract：The 45-day-old juvenile Schizothorax o′connori with total length of (1.59±0.06) cm was placed in a white plastic basin of 40 cm long× 25 cm wide × 15 cm high, exposed to 0 (control group), 25, 50, 75, 100 and 125 mg/L of alizarin red S were immersed for 24 h at water temperature of 12~13 °C for 45 days, and the mortality, growth, labeling effect and effective storage time of juvenile fish in each experimental group were explored to search suitable alizarin red labeling conditions for juvenile S. o′connori in immersion. The results showed that the median lethal concentration of alizarine red S was 225.85 mg/L for 24 h, 147.72 mg/L for 48 h, 139.21 for 72 h and 137.66 mg/L for 96 h, with the safe concentration of 28.99 mg/L. The marking of otolith was visible at the concentration of 75—125 mg/L under the alizarine red S solution of 0—125 mg/L for 24 h, with significantly increase in mortality rate of juvenile S. o′connori at alizarine red S concentration of 125 mg/L (P<0.05). Under the condition of 75 mg/L alizarine red S immersion from 12 to 96 h, the otolith marking effect of immersion from 24 to 96 h was visible, with significan tincrease in mortality rate of juvenile S. o′connori at immersion for 96 h (P<0.05). After 4 months of continuous cultivation, the otolith marking effect of juvenile S. o′connori was still visible after immersion in 75 mg/L alizarine red S solution for 72—84 h for 4 months. Considering the growth condition, survival rate, marking effect and marking preservation time, it is suggested to use 75 mg/L of alizarine red S in immersion the juvenile S. o′connori for 72—84 h.
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2024, Vol. 43 
