Cloning and Expression Analysis of Myocardial Globulin Binding Protein C3 (MyBPC3) of Jinbian Carp Cypinus carpio
WEI Lingjing1, LYU Yejian1, GAN Baojiang1, HUANG Jie2, LIU Kang1, ZHANG Sheng1, TENG Zhongzuo1, MO Feilong1, YE Xiangchen1
1. Aquatic Species Introduction and Breeding Center of Guangxi Zhuang Autonomous Region, Nanning 530031, China; 2. Fisheries Technology Extension Station of Liuzhou, Liuzhou 545006, China
Abstract：In this study, the structure and organizational expression characteristics of myosin binding protein C3 (MyBPC3) gene were investigated in Jinbian carp Cypinus carpio with body weight of (74.74±0.92) g. Functions of MyBPC3 on regulation mechanism of skin color variation were analyzed. The full-length cDNA sequence of MyBPC3 gene was cloned by RACE technique, and its tissue expression and correlation with melanin synthesis related genes (TYR, TYRP1, TYRP2 and MC-1R) were analyzed. The results showed that the full length cDNA of MyBPC3 gene was 4253 bp, including an open reading frame (ORF, 3783 bp) encoding 1260 amino acids. The predicted protein had molecular weight of 141.57 ku, the theoretical isoelectric point of 6.42, and 22 PKC and 5 PKA potential phosphorylation sites. Phylogenetic tree and homology analysis showed that the MyBPC3 gene of Jinbian carp was the most closely related to that of crucian carp Carassius auratus. The qRT-PCR results indicate that MyBPC3 mRNA is distributed in different tissues of Jinbian carp, with significantly higher expression in heart than that in brain, skin, muscle, spleen, liver, gut, and kidney tissues, and significantly lower expression level in heart and skin tissues of the carp with yellow skin individuals than that in the black one (P<0.05). The correlation analysis revealed that the MyBPC3 gene was significant positively correlated with melanin synthesis related genes (TYR, TYRP1, TYRP2 and MC-1R). In conclusion, MyBPC3 gene might involve in melanin synthesis of the skin, and its expression difference might be the cause of the skin color variation of Jinbian carp, which provided a reference for the study of the differential regulation mechanism of skin pigmentation in Jinbian carp.
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2023, Vol. 42 
