Cloning and Expression of FcBMP-7 Gene in Chinese Shrimp Fenneropenaeus chinensis
GAO Na1,2,3, LU Xia4, YAN Yunjun2,3, KONG Jie2,3, MENG Xianhong2,3, LI Xupeng2,3, SUI Juan2,3, CHEN Baolong2,3, CAO Baoxiang2,3, GAO Huan1, LUAN Sheng2,3
1. Jiangsu Key Laboratory of Marine Biotechnology, School of Marine Science and Fisheries, Jiangsu Ocean University, Lianyungang 222005, China; 2. Key Laboratory for Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 3. Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266071, China; 4. Research and Development Center for Efficient Utilization of Coastal Biological Resources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China
Abstract：In order to analyze the expression of bone morphogenetic protein-7 (BMP-7) gene, a member of transforming growth factor beta (TGF-β) superfamily, in the muscle growth and development of Chinese shrimp Fenneropenaeus chinensis, the BMP-7 (FcBMP-7) was cloned by through Rapid Amplification of cDNA Ends (RACE) technology. The full-length cDNA of FcBMP-7 was 2003 bp, containing 466 bp of 5′untranslated region(UTR), 295 bp of 3′ UTR and 1242 bp of open reading frame(ORF), encoding 413 amino acids. Using real-time PCR technology to analyze the expression pattern of FcBMP-7 in the muscle tissues of Chinese shrimp larvae at five different developmental stages (gastrulation stage, nauplius stage, zoea stage, mysis stage, and postlarval stage). The results showed that the gene was expressed in all stages of larval development, with the minimal expression in the gastrula stage, the significant increase in expression level from the nauplius stage (P<0.05), and the maximal expression level in the mysis stage; indicating that FcBMP-7 was involved in the development of shrimp muscle. Expression pattern of FcBMP-7 in 8 tissues of two sizes of Chinese shrimp revealed that FcBMP-7 had extensive tissue expression. There were significantly different in the expression levels in most tissues between the two groups (P<0.05), especially in the heart, intestine, muscle, periopod and pleopod (P<0.01). The findings suggested that FcBMP-7 gene played a certain role in the muscle growth and development of Chinese shrimp, which would lay a theoretical foundation for further analysis of the molecular mechanism of muscle development in Chinese shrimp.
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2023, Vol. 42 
