Optimization of Culture Conditions for Amplification of Female Gametophytes in Saccharina sculpera
LI Yan1,2, CHEN Shuxiu1,3, TIAN Pingping1,3, WU Ruina1,3, SAI Shan1,2, LI Xiaojie1,2
1. Shandong Oriental Ocean Group Co., Ltd., Yantai 264003, China; 2. National Algae and Sea Cucumber Engineering Technology Research Center, Yantai 264000, China; 3. Shandong Key Laboratory of Seawater Seed Industry(Constructed), Yantai 264000, China
Abstract：In order to inhibit the parthenogenesis of female gametophytes in Saccharina sculpera under conventional culture conditions of 10 ℃, light intensity of 3000—4000 lx, available nitrogen and phosphorus concentrations of 10 mg/L and 1 mg/L, the female gametophytes of the seaweed filtered through a 60 μm sieve were evenly poured into a Petri dish with a diameter of 9 cm at temperatures of 5, 8, 12, 15 and 20 ℃, the light intensity of 500. 1000, 2000, 3000 and 4000 lx, NaNO3-N concentration from 0.1 to 10.0 mg/L and KH2PO4-P concentration from 0.01 to 1.00 mg/L, aiming to improve the growth rate of female gametophytes and thus to inhibit parthenogenesis by adjusting the environmental conditions. The results showed that there were significant effects of light intensity, temperature, nitrogen and phosphorus concentrations and nitrogen source on the growth of female gametophytes in S. sculpera the seaweed. The parthenogenesis was found to be inhibited at 15 ℃, with significantly higher relative growth rate than that in other groups. The relative growth rate of female gametophytes was decreased, and the number of sporophytes produced by parthenogenesis increased significantly with the increase in light intensity. The maximal relative growth rate of female gametophytes was observed at 1000 lx, with significantly less sporophytes per unit area (1 cm2) than that in high light intensity groups (2000—4000 lx). The maximal relative growth rate (9.25%) and no parthenogenesis were found in the female gametophyte exposed to concentration of 1 mg/L in N and 0.1 mg/L in P. More and more branches in the clusters of female gametophyte cells were observed in NaNO3 and CH4N2O groups, with fewer branches of cells clustered into clusters and significantly higher relative growth rates in the other three groups. Therefore, S.sculpera grows fastest and is not prone to parthenogenesis under the conditions of white light, 1000 lx, 24 h/d, 15 ℃, 1.0 mg/L in N and 0.10 mg/L in P, and NaNO3 and CH4N2O as nitrogen source.
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2025, Vol. 44 
