Edible Yield and Proximate Composition Analysis of Wild Male Adult Mitten Crab, Eriocheir sp. in Northern China
ZU Lu1, YU Qiang1, JIANG Xiaodong1, CHENG Yongxu1,2,3, WU Xugan1,2,3
1.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 2.Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; 3.National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：The tissue coefficient, total edible yield (TEY), proximate composition and fatty acid composition of the edible tissues were investigated in wild male adult mitten handed crab, Eriocheir sp with body weight of 80—160 g collected from Suifen River, Tumen River and Liaohe River in October 2017. Results showed that there were significantly differences in gonadosomatic index (GSI), hepatosomatic index (HIS) and meat yield (MY) among the three wild populations (P<0.05), without significant difference in TEY among the three geographical populations (P>0.05). There was the maximal moisture in the gonads, in the Tumen River population, maximal lipid content in the Suifen River population, and the maximal protein and ash content in the Liaohe River population (P<0.05). The crab in the Liaohe River population had significantly higher hepatopancreatic moisture than the other two geographical populations did, and significantly lower ash content than the crab in the other two populations did (P<0.05). There was significantly higher lipid content in the hepatopancreas in the Suifen River population than that in the other two populations (P<0.05). The crab in the Suifen River population had the minimal moisture and the maximal protein, lipid and ash contents in the muscle. The minimal lipid content was observed in the muscle in the crab in the Tumen River population, and the minimal protein and ash contents in muscle tissues was found in the Liaohe River population. There was the maximal total long chain polyunsaturated fatty acid (∑LC-PUFA) content in the testis in the Suifen River population, while there were the minimal ∑LC-PUFA contents in both hepatopancreas and muscle tissues in the Tumen River population (P<0.05). The minimal n-3/n-6 value in edible tissues was found in the Liaohe River population, and the maximal n-3/n-6 value in edible tissues in the Tumen River population. In conclusion, there is no significant difference in the mean TEY of the males among the three wild populations, with significant differences in proximate composition and fatty acid contents in edible tissues among the three different geographical populations.
[1]Sui L Y, Zhang F M, Wang X M, et al. Genetic diversity and population structure of the Chinese mitten crab Eriocheir sinensis in its native range [J]. Marine Biology,2009,156(8):1573-1583. [2]刘青,刘皓,吴旭干,等.长江、黄河和辽河水系中华绒螯蟹野生和养殖群体遗传变异的微卫星分析[J].海洋与湖沼,2015,46(4):958-968. [3]Wu X G, Zhao H L,Jiang X D, et al. Comparison of culture performance and gonadal development of wild -caught Chinese mitten crab (Eriocheir sinensis) juveniles from three major river populations[J].Fisheries Science,2018,84(6):929-937. [4]Zhang C, Li Q Q, Wu X G, et al. Genetic diversity and genetic structure of farmed and wild Chinese mitten crab (Eriocheir sinensis) populations from three major basins by mitochondrial DNA COⅠ and Cyt b gene sequences[J]. Mitochondrial DNA Part A,2017,29(7):1081-1089. [5]农业农村部渔业渔政管理局.2019中国渔业统计年鉴[M].北京:中国农业出版社,2019. [6]Chu K H, Ho H Y, Li C P,et al. Molecular phylogenetics of the mitten crab species in Eriocheir, sensu lato (Brachyura: Grapsidae) [J]. Journal of Crustacean Biology,2003,23(3):738-746. [7]Tang B P, Zhou K Y, Song D X, et al. Molecular systematics of the Asian mitten crabs, genus Eriocheir, (Crustacea: Brachyura) [J]. Molecular Phylogenetics and Evolution,2003,29(2):309-316. [8]Xu J W, Chu K H. Genome scan of the mitten crab Eriocheir sensu stricto in East Asia: population differentiation, hybridization and adaptive speciation[J]. Molecular Phylogenetics and Evolution,2012,64(1):118-129. [9]Kang W, Huang S, Chen X W, et al. Genetic admixture of mitten crabs in the Northeast Asia hybrid zones[J]. Hydrobiologia,2018,806(1):203-214. [10]徐洁,王晓梅,李晶晶,等.基于COⅠ序列绒螯蟹属DNA条形码和遗传多样性研究[J].水产科学,2017,36(4):480-487. [11]吴旭干,龙晓文,刘乃更,等.中华绒螯蟹、日本绒螯蟹及其杂交种性腺发育和生化组成的比较研究[J].淡水渔业,2015,45(3):3-8. [12]卢义,吴旭干,何杰,等.长江、黄河、辽河水系中华绒螯蟹野生扣蟹的形态学及生化组成[J].中国水产科学,2016,23(2):382-395. [13]赵恒亮,吴旭干,龙晓文,等.长江、黄河和辽河种群中华绒螯蟹雄体成蟹可食组织营养组成的比较[J]. 中国水产科学,2016,23(5):1117-1129. [14]张成,祖露,李清清,等.基于脂肪酸生物标记法探讨中国沿海六群体绒螯蟹(Eriocheir sensu stricto)的种群遗传[J]. 海洋与湖沼,2019,50(2):388-397. [15]康伟,吴廉,刘金生,等.图们江水系绒螯蟹的形态差异与遗传混杂[J].中国水产科学,2016,23(3):555-564. [16]Wu X G, Cheng Y X, Sui L Y, et al. Biochemical composition of pond-reared and lake-stocked Chinese mitten crab Eriocheir sinensis (H. Milne-Edwards) broodstock [J]. Aquaculture Research,2007,38(14):1459-1467. [17]何杰,吴旭干,龙晓文,等.长江水系中华绒螯蟹野生和养殖群体选育子一代养殖性能和性腺发育的比较[J]. 海洋与湖沼,2015,46(4):808-818. [18]Roynette C E, Calder P C, Dupertuis Y M, et al. N-3 polyunsaturated fatty acids and colon cancer prevention [J]. Clinical Nutrition,2004,23(2):139-151. [19]Chen D W, Zhang M,Shrestha S. Compositional characteristics and nutritional quality of Chinese mitten crab (Eriocheir sinensis) [J]. Food Chemistry,2007,103(4):1343-1349. [20]Kuley E,zogˇ[BP(ul F, zogul Y, et al. Comparison of fatty acid and proximate compositions of the body and claw of male and female blue crabs (Callinectes sapidus) from different regions of the Mediterranean coast [J]. International Journal of Food Sciences and Nutrition,2008,59 (7/8):573-580. [21]Wang Q J, Wu X G, Long X W, et al. Nutritional quality of different grades of adult male Chinese mitten crab, Eriocheir sinensis [J].Journal of Food Science and Technology,2018,55(3):944-955. [22]汪倩,吴旭干,楼宝,等.三疣梭子蟹不同部位肌肉主要营养成分分析[J].营养学报,2013,35(3):310-312. [23]Krzeczkowski R A, Stone F E. Amino acid, fatty acid and proximate composition of snow crab (Chionoecetes bairdi) [J]. Journal of Food Science,1974,39(2):386-388. [24]赵恒亮,吴旭干,姜晓东,等.池塘养殖条件下长江、黄河和辽河种群中华绒螯蟹雌体卵巢发育和营养组成的比较研究[J].水产学报,2017,41(1):109-122. [25]周加义,叶慧.冷冻干燥法和烘箱法测定肌肉中水分及对粗蛋白、粗脂肪测定的影响[J]. 中国家禽,2017,39(11):51-53. [26]AOAC.Official Methods of Analysis of the Association of Official Analytical Chemists [M].16th ed. Arlington, VA, USA: Association of Official Analytical Chemists,1995. [27]Folch J, Lees M, Sloane-Stanley G. A simple method for the isolation and purification of total lipides from animal tissues [J].Journal of Biological Chemistry,1957,226(1):497-509. [28]Morrison W R, Smith L M. Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride-methanol[J]. Journal of Lipid Research,1964,5(4):600-608. [29]成永旭,赖伟.十足类甲壳动物卵巢发育过程中脂肪的积累与肝胰腺脂肪的变化[J]. 动物学杂志,1997,32(2):57-60. [30]王武,王成辉,马旭洲.中华绒螯蟹生态养殖[M].北京:中国农业出版社,2013:59-84. [31]滕炜鸣,成永旭,吴旭干,等.莱茵种群和长江种群子一代中华绒螯蟹性腺发育及相关生物学指数变化的比较[J].上海水产大学学报,2008,17(1):65-71. [32]Wu X G, Zhou B, Cheng Y X, et al. Comparison of gender differences in biochemical composition and nutritional value of various edible parts of the blue swimmer crab[J]. Journal of Food Composition and Analysis,2010,23(2):154-159. [33]王群,赵云龙,马强,等.中华绒螯蟹(Eriocheir sinensis)雄性生殖系统发育过程中生化成分的季节变化[J]. 海洋与湖沼,2004,35(4):351-357. [34]麻楠,龙晓文,吴旭干,等.中华绒螯蟹成熟雄体生殖系统的生化组成分析[J].上海海洋大学学报,2017,26(6):872-879. [35]卢义.三水系中华绒螯蟹(Eriocheir sinensis)形态特征、生化组成和养殖性能的比较研究[D].上海:上海洋洋大学,2014. [36]瞿俐俐,王锡昌,吴旭干,等.影响蟹类营养品质主要因素的研究进展[J]. 食品工业,2017,38(3):217-221. [37]Wu X G, Wang Z K, Cheng Y X, et al. Effects of dietary phospholipids and highly unsaturated fatty acids on the precocity, survival, growth and hepatic lipid composition of juvenile Chinese mitten crab, Eriocheir sinensis (H. Milne-Edwards)[J]. Aquaculture Research,2011,42(3):457-468. [38]陈丽花,肖作兵,周培根.中国对虾的脂肪酸分析及其营养价值评价[J].上海海洋大学学报,2010,19(1):125-129. [39]王维群.营养学[M].北京:高等教育出版社,2005. [40]elik M, Türeli C, elik M, et al. Fatty acid composition of the blue crab (Callinectes sapidus Rathbun) in the north eastern Mediterranean [J]. Food Chemistry,2004,88(2):271-273. [41]Innis S M. The role of dietary n-6 and n-3 fatty acids in the developing brain[J]. Developmental Neuroscience,2000,22(5/6):474-480. [42]FAO/WHO. Fats and oils in human nutrition. Report of a Joint FAO/WHO Expert Consultation [R].Geneva:WHO,1993:19-26.
2021, Vol. 40 
