Effects of Allicin on Growth and Disease Resistance in Turbot
LI Huitao1,2, HUANG Bin1, LIU Baoliang1, LIU Bin1, WANG Weifang1
1. Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; 2. Shandong Baifu Biotech Co, Ltd, Jining 273200, China
Abstract：Juvenile turbot (Scophthalmus maximus) with body weight of (8.12±0.03) g was reared in cement ponds of each 5.0 m × 5.0 m × 0.6 m at stocking density of 3600 individuals per pond and fed diets containing 0 (control), 100, 200, 400, and 800 mg/kg diet allicin at water temperature of 13—15 ℃ and a salinity of 30 for 6 weeks. At the end of the feeding trial, pathogen Vibrio anguillarum was injected intraperitoneally into the turbot for 14 days to infect; after 24 hours of starvation, the sample fish was weighed and blood was collected from caudal peduncle vein to determine the content of complement C3, and the activities of lysozyme and superoxide dismutase (SOD) in serum to explore the effects of allicin on the growth, non-specific immunity and disease resistance of turbot. It was found that the specific growth rate was increased with the increasing level of dietary allicin, significantly higher in the juveniles fed diets containing allicin up to 200 mg/kg and over than that in the juveniles in control group (P<0.05). The fish fed control diet had significantly lower survival rate than the fish in other groups did (P<0.05). The food conversion ratio was shown to be decreased with the increase in dietary allicin, significantly lower than that in the 100 mg/kg group, and stable in the dietary allicin up to 200 mg/kg group. Serum complement C3 contents were increased with the increasing level of dietary allicin from 0 to 400 mg/kg, with the maximal C3 contents in 200 and 400 mg/kg groups, significantly higher than any other groups (P<0.05). The lysozyme and SOD activities in serum were found to be increased with the increasing level of dietary allicin, without significant increase in dietary allicin up to 400 mg/kg group. The challenge test indicated that the infection mortalities in treatment of 400 and 800 mg allicin/kg diet were significantly lower than that in any other treatments (P<0.05). Therefore, it is recommended that 200 mg/kg diet be essential to maintain the optimal growth for the juvenile turbot, and 400 mg/kg diet leads to improve nonspecific immunity and ability to fight enteritis more effectively.
李会涛, 黄滨, 刘宝良, 刘滨, 王蔚芳. 大蒜素对大菱鲆幼鱼生长及抗病力的影响[J]. 水产科学, 2020, 39(4): 539-544.
LI Huitao, HUANG Bin, LIU Baoliang, LIU Bin, WANG Weifang. Effects of Allicin on Growth and Disease Resistance in Turbot. Fisheries Science, 2020, 39(4): 539-544.
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2020, Vol. 39 
