Characterization and Expression Profiles of Three Interleukin Genes in Tiger Puffer Takifugu rubripes
YANG Xu1, WU Dongshu1, HUA Xintong1, LI Rong2, LYU Wei2, JIANG Chen1
1.Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116023, China; 2.Dalian Fugu Limited Group, Dalian 116400, China
Abstract：The sequence characteristics of the interleukin genes IL-1b, IL-8 and IL-10 were investigated in tiger puffer Takifugu rubripes with body weight of (190.34±2.13) g, and the expression of interleukin genes was detected in the brain, gills, heart, muscle, liver and spleen of tiger puffer. Changes in expression level of the interleukin genes IL-1b, IL-8 and IL-10 in liver and spleen were monitored in tiger puffer injected with Vibrio harveyi solution at a dose of 0.1 mL/individual at a concentration of 1×107 cells/mL 0 h, 12 h, 24 h, and 48 h after administration. It was found that the sequence length was 771 bp in IL-1b, 822 bp in IL-8 and 844 bp in IL-10. The three genes were all expressed in heart, brain, gills, muscle, liver, and spleen of normal tiger puffer, significantly higher expression levels in the liver and spleen (P<0.05). In the challenged tiger puffer, IL-1b gene was shown to be significantly elevated expression level in the liver at 12 h, and in the spleen at 12 h and 48 h post infection with V. harveyi (P<0.05). IL-8 gene expression level was increased significantly in the liver and the spleen at 12 h and 24 h (P<0.05); while IL-10 gene was shown to be significantly expressed in the liver at 24 h, and 48 h, and spleen at 12 h and 48 h (P<0.05). The analysis of sequence characteristics and expression profiles of IL-1b, IL-8 and IL-10 genes in tiger puffer for the first time provided experimental information for the prokaryotic expression and recombinant proteins activities study of these genes, and the foundation for the application of healthy cultivation of tiger puffer.
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2021, Vol. 40 
