Analysis of Growth, Body Color and Expression Levels of Skin Color Related Genes in Red Family Common Carp Cyprinus carpio FFRC No.2 Strain
WAN Shunpeng1, ZHU Wenbin2, WANG Lanmei2, LUO Mingkun2, FU Jianjun2, DU Shumei3, DONG Zaijie1,2
1. Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214128, China; 2. Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs; Freshwater Fisheries Research Centre, Chinese Academy of Fishery Sciences, Wuxi 214081, China; 3. Nanchang Academy of Agricultural Sciences, Nanchang 330038, China
Abstract：In order to investigate the differences in body color differentiation and growth in the red FFRC No.2 strain common carp Cyprinus carpio , the fry from two families of FFRC No. 2 strain including red A and B families bred by multi-generations, forming 4 complete diallel crossing combinations of AA, BB, AB, and BA, with 4 families for each combination, were reared in 16 cages of 1m × 1m × 1m placed in the same pond for 2 months. Then the juveniles in each family were marked by PIT and the body weight, body length, body thickness and body depth were measured. The 2 months old juveniles were cultured in the same pond for another 3 months and measured again to analyze growth, body color, enzyme related to pigment synthesis activities and the expression level of body color related genes in the offspring in each group. The results showed that the offspring in AA and BA groups were all red in skin color, with significant advantage in body weight, length, thickness, and depth in the BA group compared to the other three groups, while the offspring in BB and AB groups had both all red and red green-backed (with green-grey stripes on the back) phenotypes, with having 80.1 % of green-backed/total in BB group and 87.3 % green-backed/total in AB group. It was found that the green-backed individual had considerably higher body weight, length, thickness, and depth than those in the all-red ones (P<0.05), without significant difference in activities of GTP cyclohydrolase 1 (Gch1) and tyrosinase (Tyr) in the four groups (P > 0.05). There was significantly higher relative expression level of cyclic hydrolase 1 (Gch1) gene in dorsal skin tissue in AA group than that in the BB and AB groups (P<0.05), and there were significantly higher relative expression levels of xanthine dehydrogenase (Xdh) gene and murine reductase (Spr) gene in the AA group than those in BB, AB, and BA groups (P＜0.05). Tyrosinase (Tyr) and pre-melanocortin gene (Pmel) expression levels were significally higher in the AA, AB, and BA groups than those in BB group (P<0.05), without statistically different melanocortin 1 receptor gene (Mc1r) expression levels between groups (P > 0.05). Group AA common carp, FFRC No.2 strain can be utilized as a basis material to supply parents for the next generation of selection, according to a combination of indicators. The findings explored the law of body color differentiation of the red, FFRC No.2 strain, and provided certain basic research data for further cultivating red carp with good growth and body color.
万顺鹏, 朱文彬, 王兰梅, 罗明坤, 傅建军, 杜淑玫, 董在杰. 红福瑞鲤2号生长、体色及相关基因表达分析[J]. 水产科学, 2023, 42(6): 1032-1039.
WAN Shunpeng, ZHU Wenbin, WANG Lanmei, LUO Mingkun, FU Jianjun, DU Shumei, DONG Zaijie. Analysis of Growth, Body Color and Expression Levels of Skin Color Related Genes in Red Family Common Carp Cyprinus carpio FFRC No.2 Strain. 水产科学, 2023, 42(6): 1032-1039.
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2023, Vol. 42 
