Structural and Functional Characteristics of Intestinal Microbiota in Different Populations of Macrobrachium rosenbergii Larvae
SHI Jingu1,2, WU Xia1,3, HUANG Guanghua1, YANG Huizan1, LIANG Yi2, LYU Min1, ZENG Lan1, HU Dasheng2, HUANG Libin1, WANG Rui1
1. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China; 2. Fisheries Technology Extension Center of Guangxi, Nanning 530022, China; 3. College of Life Sciences, Guangxi Normal University, Guilin 541006, China
Abstract：Under the same culture conditions, five populations of Macrobrachium rosenbergii were cultured. The larvae of M. rosenbergii hatched for 20 days in the same batch were selected. The intestinal microflora of the larvae of Burmese population, Sri Lankan population, Vietnamese population, Bengali population and own variety 1 population were analyzed by 16S rRNA high-throughput sequencing technology. The results showed that there were 256 OTUs in common in five groups, and the unique OTUs of each group were 131, 247, 231, 144 and 184, respectively. The species richness of intestinal microbiota in Bengali population was the highest, and the species diversity of gut microbiota in Vietnamese population was the highest. The dominant microflora of five groups were similar in phylum and family level, which were Proteobacteria, Bacteroidetes and Firmicutes, but their abundance and composition of other microflora were different. The abundance of Firmicutes in intestinal microflora of Sri Lankan population was higher than that of Bacteroidetes, which was different from the other four groups. The five groups also have common functions, mainly focusing on carbohydrate metabolism, amino acid metabolism, vitamin metabolism and so on. The results showed that the intestinal microbiota of different populations of M. rosenbergii had some similarities in structure, abundance and metabolic function. This study analyzed the structure and functional characteristics of intestinal microbiota of different populations of M. rosenbergii larvae, which laid a foundation for further study on the growth and development mechanism of different populations of M. rosenbergii.
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2023, Vol. 42 
