Effects of Dietary Selenium Yeast on Antioxidant Capacity, Immune Response, and Digestive Enzyme Activities in Manila Clam Ruditapes philippinarum
LU Yanfeng1,2, XIONG Yuyang1,2, HUO Zhongming1,2, GAO Zhiying1,2, WANG Lianshun1, DING Jianfeng1,2
1. College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2. Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province, Dalian 116023, China
Abstract：To investigate the effects of different concentrations of selenium yeast on the antioxidant capacity, immune function, and digestion of Manila clam Ruditapes philippinarum, healthy clams with initial shell length of (32.49±2.50) mm and body weight of (8.16±1.81) g were cultured in 20 L tanks at water temperature of (20±1) °C and salinity of 31±1, and fed with selenium yeast powder (selenium content 6500 mg/kg) at final mass concentrations of 0 (control), 2, 4, 6, and 8 mg/L for 30 days. Activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM), and malondialdehyde (MDA), as well as total antioxidant capacity (T-AOC) in the hemolymph and hepatopancreas, along with the activities of α-amylase, trypsin, and lipase in the hepatopancreas, were determined at the end of the experiment. The results showed that: (1) SOD activity and T-AOC in hemolymph in the 8 mg/L group, CAT activity in the 2 mg/L group, and GSH-Px activity in the 6 mg/L group were significantly increased (P＜0.05). In the hepatopancreas, CAT activity in the 4 mg/L group, and GSH-Px activities in the 6 and 8 mg/L groups were significantly increased (P＜0.05), with the maximal SOD activity and T-AOC in the 4 mg/L group. (2) The maximal ACP activity in hemolymph was observed in the 4 mg/L group (P＜0.05), with significant increase in the 8 mg/L group (P＜0.05). LZM activity in hemolymph was significantly increased in the 2, 6, and 8 mg/L groups (P＜0.05). In the hepatopancreas, ACP and LZM activities in the 4 mg/L group were significantly increased (P＜0.05). (3) There were significant increases in trypsin activity in the 8 mg/L group and significant decrease in lipase activities in the hepatopancreas in the 2—6 mg/L groups (P＜0.05), without significant difference in α-amylase activity. In conclusion, appropriate supplementation of selenium yeast can significantly regulate the antioxidant and immune functions and affect digestive enzyme activities in the hepatopancreas of Manila clam. The findings provide a foundation for the healthy aquaculture of the clam.
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2026, Vol. 45 
