Abstract：A study was carried out to test the effect of acute mercuric chloride (HgCl2) exposure on Pacific white shrimp Litopenaeus vannamei. In order to investigate the acute toxic effect of HgCl2 on Pacific white shrimp, the juvenile Pacific white shrimp with body weight of (5.01±0.06) g was placed in a 40 L glass tank (54 cm× 40 cm× 30 cm) at a water temperature of 27—29 °C at a rate of 40 individuals per tank and exposed to HgCl2 at concentration of < 0.001 mg/L as the control group, 0.033 mg/L and 0.33 mg/L group. The plasma antioxidant enzyme activity, total antioxidant capacity and immune response indices and Hg content in the muscle were determined at 24 h, 48 h, 72 h and 96 h. The results showed that there was the maximal Hg content in muscle in the shrimp in 0.33 mg/L HgCl2 group after 24 h (P>0.05). There were higher total antioxidant capacity and superoxide dismutase activity in plasma of shrimp in 0.033 and 0.33 mg/L HgCl2 groups than those in shrimp in control group at 24 h (P>0.05). The lower superoxide dismutase and catalase activities were observed in plasma of shrimp in 0.33 mg/L HgCl2 group than those in shrimp in 0.033 mg/L HgCl2 group after 72 h, with the maximal malondialdehyde content (P>0.05). The maximal activities of acid phosphatase, alkaline phosphatase, lysozyme and phenol oxidase were shown in plasma of shrimp in 0.33 mg/L HgCl2 group at 24 h (P<0.05). In 48 h, the acid phosphatase and lysozyme activities in plasma of shrimp in 0.33 mg/L HgCl2 group were found to be the minimum (P<0.05). The contents of interleukin 1 and 8 in plasma of shrimp in 0.33 mg/L HgCl2 group were the maximal after 24 h (P<0.05). The findings showed that the Pacific white shrimp exposed to HgCl2 led to accumulation of Hg in muscle and malondialdehyde in blood, reducing antioxidant enzyme activity, causing immunosuppression and inflammation.
董炜峰, 杨毕铖, 李光毅, 戴红, 刘志勇, 蒋灏. 不同质量浓度HgCl2对凡纳滨对虾的急性毒性评估[J]. 水产科学, 2024, 43(1): 97-103.
DONG Weifeng, YANG Bicheng, LI Guangyi, DAI Hong, LIU Zhiyong, JIANG Hao. Acute Toxicity Assessment in Pacific White Shrimp Exposed to Different Concentrations of Mercuric Chloride. Fisheries Science, 2024, 43(1): 97-103.
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2024, Vol. 43 
