Genetic Polymorphism Analysis of Cytb and COⅠ Gene Sequences of Icefish Protosalanx hyalocranius and Neosalanx taihuensis in Lake Gaoyou
LI Daming1, SUN Wenxiang2, XU Fei2, TANG Shengkai1, LIU Yanshan1, GU Xiankun1, LIU Xiaowei1, ZHANG Tongqing1
1.Key Laboratory of Fisheries Resources in Inland Waters of Jiangsu Province, Freshwater Fisheries ResearchInstitute of Jiangsu Province, Nanjing 210097, China; 2.Gaobaoshaobohu Lake Fisheries AdministrationCommittee Office of Jiangsu Province, Yangzhou 225009, China
Abstract：The genetic diversity and genetic structure were analyzed in two populations of icefish Protosalanx hyalocranius and Neosalanx taihuensis in Lake Gaoyou by sequencing the Cytb and COⅠ genes in the mitochondrial DNA in order to understand the current situation of wild resources of the wild populations of icefish in the Lake Gaoyou. The full Cytb gene sequences (1141 bp) were obtained from 40 individuals of P.hyalocranius,14 variable sites were detected and 12 haplotypes were defined, with haplotype diversity of 0.871±0.031, and the nucleotide diversity of 0.00172±0.00019, showing a type of high haplotype diversity and low nucleotide diversity. The partial COⅠ gene sequences (630 bp) were obtained, 5 variable sites were detected and 6 haplotypes were defined in 40 individuals of P.hyalocranius, with haplotype diversity of 0.747±0.041, and the nucleotide diversity of 0.00202±0.00019, showing a type of high haplotype diversity and low nucleotide diversity. The full Cytb gene sequences (1141 bp) were obtained,13 variable sites were detected and 9 haplotypes were defined from 40 individuals of N. taihuensis, with haplotype diversity of 0.609±0.078, and nucleotide diversity of 0.00094±0.00027, which had a type of high haplotype diversity and low nucleotide diversity. The partial COⅠ gene sequences (630 bp) were obtained, 2 variable sites were detected and 3 haplotypes were defined in 40 individuals of N. taihuensis, with haplotype diversity of 0.232±0.085, and nucleotide diversity of 0.00038±0.00014, indicating that it had a type of low haplotype diversity and low nucleotide diversity. The neutrality test revealed that icefish P. hyalocranius and N. taihuensis in Lake Gaoyou had negative Tajima′s D and Fu′s Fs values, with significant difference, and mismatch distribution analysis map showed a peak type, suggesting P. hyalocranius and N. taihuensis have experienced population expansion in the history. The findings indicate that it should take much more measures to protect the icefish resources in Lake Gaoyou.
[1]解玉浩,解涵. 银鱼科鱼类的分类分布和种群生态[J]. 水产学杂志,1997,10(2):11-19. [2]王忠锁,傅萃长,雷光春. 中国银鱼的多样性及其保护对策[J]. 生物多样性,2002,10(4):416-424. [3]顾良伟. 我国太湖银鱼移植现状[J]. 现代渔业信息,1998,13(9):23-25. [4]秦伟,责文方. 我国湖泊水库银鱼移植增殖生态及技术[J]. 上海水产大学学报,1999,8(4):358-370. [5]孙帼英. 大银鱼卵巢的成熟期和产卵类型[J]. 水产学报,1985,9(4):363-368. [6]解玉浩. 大银鱼的繁殖生物学特性及移植放流的主要措施[J]. 水利渔业,1996(1):6-8. [7]王玉芬,蒋全文. 太湖大银鱼生长特性的研究[J]. 湖泊科学,1992,4(1):56-62. [8]邵晓阳,黎道峰,潭路,等. 三峡库区银鱼生长特点及资源分析[J]. 生态学报,2011,31(17):4858-4865. [9]周彦锋,徐东坡,单俊峰,等. 3个地理群体大银鱼营养成分的分析与评价[J]. 上海海洋大学学报,2011,20(5):734-740. [10]吕国庆,李思发. 鱼类线粒体DNA 多态研究和应用进展[J]. 中国水产科学,1998,5(3):94-99. [11]孙鹏,彭士明,尹飞,等. 利用COⅠ和Cytb序列探讨东海区3种鲳属鱼类的种群遗传结构[J]. 海洋渔业,2011,33(4):398-404. [12]李大命,李康,张彤晴,等. 洪泽湖大银鱼(Protosalanx hyalocranius) Cytb和COⅠ基因序列多态性分析[J].渔业科学进展,2017,38(6):25-31. [13]李青,王波,郑风荣,等.星斑川鲽、黄盖鲽和石鲽线粒体基因Cytb和COⅠ片段序列的比较研究[J].水产科学,2016,35(6):625-632. [14]王学武,孙羊林,胡恒元,等. 高邮湖湿地保护与利用[J]. 湿地科学与管理,2010,6(3):38-40. [15]段斌. 高邮湖渔业资源开发利用与保护研究[D].扬州:扬州大学,2015. [16]朱银安,单红,王庆,等. 长江、高邮湖、太湖日本沼虾遗传多样性的RAPD分析[J]. 水产养殖,2008(1):5-7. [17]李大命,张彤晴,关浩勇,等. 基于线粒体Cytb基因和D-loop区序列的高邮湖湖鲚(Coilia nasus)遗传多样性分析[J]. 淡水渔业,2017,47(6):3-8. [18]Zhang J, Li M, Xu M Q, et al. Molecular phylogeny of icefish Salangidae based on complete mtDNA cytochromeb sequences, with comments on estuarine fish evolution[J]. Biological Journal of the Linnean Society,2007,91(2):325-340. [19]Ward R D, Zemlak T S, Innes B H, et al. DNA barcoding Australia′s fish species[J]. Philosophical Transactions of the Royal Society of London,2005,360(1462):1847-1857. [20]Hall T A. BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT[J]. Nucleic Acids Symposium Series,1999,41:95-98. [21]Kumar S,Stecher G, Tamura K. MEGA7:Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2016,33(7):1870-1874. [22]Librado P, Rozas J. DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics,2009,25(11):1451-1452. [23]Excoffier L, Lischer H E L. Arlequin suite ver 3.5:a new series of programs to perform population genetics analyses under Linux and Window[J]. Molecular Ecology Resources,2010,10(3):564-567. [24]Feral J P. How useful are the genetic markers in attempts to understand and manage marine biodiversity[J]. Journal of Experimental Marine Biology and Ecology,2002,268(2):121-145. [25]Grant W A S, Bowen B W. Shallow population histories in deep evolutionary lineages of marine fishes:insights from sardines and anchovies and lessons for conservation[J]. Journal of Heredity,1998,89(5):415-426. [26]罗宏伟,段辛斌,王珂,等. 三峡库区3种银鱼线粒体DNA细胞色素b基因序列多态性分析[J]. 淡水渔业,2009,39(6):16-21. [27]李大命,张彤晴,唐晟凯,等,太湖大银鱼(Protosalanx chinensis)细胞色素b基因序列多态性分析[J]. 江苏农业学报,2015,31(4):840-845. [28]薛丹. 基于细胞色素b的中国9个大银鱼群体遗传多样性研究[D]. 广州:暨南大学,2015. [29]Tang F J, Li D M, Liu W, et al. Evolutionary tendency of clearhead icefish Protosalanx hyalocranius inferring mitochondrial DNA variation analyses in Amur (Heilongjiang) River catchment, China[J]. International Journal of Agriculture & Biological,2018,20(10):2329-2334. [30]张迪,雷光春,龚成,等. 基于COⅠ基因序列的太湖新银鱼遗传多样性[J]. 湖泊科学,2012,24(2):299-306. [31]Zhao L, Zhang J, Liu Z J, et al. Complex population genetic and demographic history of the salangid, Neosalanx taihuensis, based on cytochrome b sequences[J]. BMC Evolutionary Biology,2008,8:201. [32]毕潇潇,高天翔,肖永双,等. 4种鳕鱼线粒体16S rRNA、COⅠ和Cytb基因片段序列的比较研究[J]. 南方水产,2009,5(3):46-52. [33]孙鹏,彭士明,尹飞,等. 利用COⅠ和Cytb 序列探讨东海区3 种鲳属鱼类的种群遗传结构[J]. 海洋渔业,2011,33(4):398-404. [34]胡静,侯新远,尹绍武,等. 基于mtDNA COⅠ和 Cytb 基因序列对南中国海不同海域波纹唇鱼群体遗传多样性的研究[J]. 水生生物学报,2014,38(6):1008-1016. [35]Tajima F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism [J]. Genetics,1989,123(3):585-595. [36]Fu Y X. Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection[J]. Genetics,1997,147(2):915-925.
2020, Vol. 39 
