Analysis of Difference in Intestinal Bacterial Flora between Common Carp Cyprinus carpio var. jinbian and C. carpio var. jian Cultured in Paddy Fields and in Ponds
YAN Xueyu1, YE Xiangchen1, WEI Lingjing1, WEN Yanhong2, YANG Zhushan3, TENG Zhongzuo1, LIU Kang1, LYU Yejian1
1. Aquatic Species Introduction and Breeding Center of Guangxi Zhuang Autonomous Region, Nanning 530031, China; 2. Fisheries Technology Extension Station of Liuzhou, Liuzhou 545006, China; 3. Fisheries Technology Extension Station of Rongshui Miao Autonomous County, Liuzhou 545300, China
Abstract：The intestinal bacterial community and relative abundance were comparatively analyzed in common carp Cyprinus carpio var. jinbian and C.carpio var.jian cultured from different modes, including the pond culture group of Jinbian carp (B1), the paddy field culture group of Jinbian carp (B2), the pond culture group of Jian carp (N1) and the field culture group of Jian carp (N2), by using MiSeq high-throughput sequencing technology to detect the hypervariable region of 16S rRNA gene. The results showed that 377, 250, 367 and 455 Operational Taxonomic Units (OTUs) in the intestine were obtained in the B1, B2, N1 and N2 groups, respectively. OTU cluster analysis revealed that the results of N2 group were the most different from those of other three groups. Diversity analysis of the intestinal bacterial community indicated that the Simpson index was significantly higher in B1 than that in B2, N1 and N2 (P<0.05), with Shannon index of 1.56±0.22 in B2 and 2.53±0.82 in N2, which were both higher than that in the corresponding pond groups, without significant difference (P>0.05). Proteobacteria (89.56%) and Aeromonas (82.68%) were the dominant bacteria in B1 group(P<0.05), and the dominant bacteria were Proteobacteria, Fusobacteriaand Fimicutes in the phylum level, and Cetobacterium, Aeromonas and Lactococcus in the genus level in B2, N1 and N2 groups. KEGG analysis showed that the intestinal bacterial in B1, B2, N1 and N2 groups involved in various metabolic pathways, signal conduction-, regulation and repair-related signaling pathways. The findings indicated that the dominant bacteria, relative abundance and involved pathways of Jinbian carp in the field-or pond- culture mode were not significantly different from those of Jian carp, suggesting that Jinbian carp could adapt to the two culture modes as other common carp.
严雪瑜, 叶香尘, 韦玲静, 文衍红, 杨著山, 滕忠作, 刘康, 吕业坚. 稻田和池塘两种模式下金边鲤与建鲤肠道菌群差异分析[J]. 水产科学, 2020, 39(4): 509-516.
YAN Xueyu, YE Xiangchen, WEI Lingjing, WEN Yanhong, YANG Zhushan, TENG Zhongzuo, LIU Kang, LYU Yejian. Analysis of Difference in Intestinal Bacterial Flora between Common Carp Cyprinus carpio var. jinbian and C. carpio var. jian Cultured in Paddy Fields and in Ponds. Fisheries Science, 2020, 39(4): 509-516.
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2020, Vol. 39 
