Effects of Different Salinities on Growth and Osmotic Regulation Gene Expression of Gymnocypris przewalskii
HUANG Shen1, LI Changzhong1, LI Zixuan1, LIU Yanhui1, LIU Fang1, QI Hongfang2, WANG Yang2, JIN Wenjie1
1.College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China; 2.Key Laboratory of Breeding and Protection of Gymnocypris przewalskii in Qinghai Province, Rescue Center of Gymnocypris przewalskii, Xining 810016, China
Abstract：Qinghai Lake is the largest inland brackish lake in China. The salinity adaptation mechanism of Gymnocypris przewalskii, a unique and rare fish species living in it, needs to be clarified urgently. Therefore, the growth performance and osmotic regulation genes of G. przewalskii were investigated after salinity stress by physiological index and qPCR and Na+/K+/2Cl- cotransporter 1 (NKCC1), Aquaporin 1 (AQP1), Na+/H+ exchange 1 (NHE1) and Na+/HCO-3 exchange 1 (NBC1) genes were detected. The results showed that there were significant differences in the growth indicators of G. przewalskii under different salinities (P<0.05), indicating that the increase in salinity affected the development of G. przewalskii, and that the growth status were negatively correlated with salinity. NKCC1, NBC1, AQP1 and NHE1 genes were expressed in the gills, kidneys and livers under different salinities, showing certain tissues specificity. It was found that that NKCC1, AQP1, NHE1 and NBC1 genes were involved in the regulation of osmotic pressure of G. przewalskii, with varying the expression level with different salinities, tissue and stress time, reflecting that the gill and kidney were the main organ regulating osmotic pressure. The findings provided a theoretical basis for exploring the survival status and salinity adaptation mechanism of G. przewalskii after migration in Qinghai Lake, and a theoretical guidance for the protection and artificial proliferation and release of native fish germplasm resources in the upper reaches of the Yellow River.
黄屾, 李长忠, 李梓瑄, 刘艳慧, 柳芳, 祁洪芳, 汪洋, 金文杰. 盐度对青海湖裸鲤生长及渗透调节基因的影响[J]. 水产科学, 2022, 41(4): 527-536.
HUANG Shen, LI Changzhong, LI Zixuan, LIU Yanhui, LIU Fang, QI Hongfang, WANG Yang, JIN Wenjie. Effects of Different Salinities on Growth and Osmotic Regulation Gene Expression of Gymnocypris przewalskii. 水产科学, 2022, 41(4): 527-536.
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2022, Vol. 41 
