Effects of Temperature on Oxygen Consumption Rate, Suffocation Threshold, and Antioxidant Capacity of Juvenile Chinese Hooksnout Carp Opsariichthys bidens
ZHANG Kai1,2, WANG Weiyu1, ZHAO Liyue1, XIONG Luomei1, MEI Yehan1, HUANG Kexin1, LIU Fangling1,2, YU Jin3, ZHENG Shanjian1,2
1. College of Chemistry and Bioscience, Zhejiang Normal University, Jinhua 321004, China; 2. Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua 321004, China; 3. Longyou County Aquaculture Development Center, Quzhou 324000, China
Abstract：In order to explore the oxygen consumption rate, suffocation threshold, and antioxidant index of two sizes of juveniles Chinese hooksnout carp Opsariichthys bidens in different cultural environments, the oxygen consumption rate, suffocation point, and anti-oxidation indices of Chinese hooksnout carp were determined under three temperature gradients of 20, 24 and 28 ℃. It was found that the oxygen consumption rate and suffocation point of the juveniles was increased first and then decreased with increasing temperature. The oxygen consumption rate and suffocation point of small-sized juveniles (1.22±0.31) g at 20 ℃ were (0.185±0.057) mg/(g·h) and (1.149±0.110) mg/L, significantly lower than the consumption at 24 ℃ and 28 ℃(P<0.05). The suffocation threshold of small-sized juveniles was significantly higher at 24 ℃ than that at 28 ℃(P<0.05), but there was no significant difference in oxygen consumption rate(P>0.05); the oxygen consumption rate of large-sized juveniles(4.91±0.55) g under three temperature gradients was 24 ℃>28 ℃>20 ℃, and the suffocation point was 28 ℃>24 ℃>20 ℃. Under the condition of 28 ℃, there was no significant difference in the oxygen consumption rate of two kinds of juvenile fish(P>0.05). Under hypoxia stress, the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and total antioxidant capacity (T-AOC) in liver tissues of the juvenile were significantly increased (P<0.05), and the SOD activity and T-AOC content of the liver of the juvenile were higher than those of the small ones (P<0.05). The findings indicate that the adaptability of small-sized juveniles to the environment is lower than that of large-sized juveniles, and they are more susceptible to damage under hypoxia and temperature stress.
[1]亢玉静,郎明远,赵文.水生生物体内抗氧化酶及其影响因素研究进展[J].微生物学杂志,2013,33(3):75-80. [2]HANDELAND S O, IMSLAND A K, STEFANSSON S O. The effect of temperature and fish size on growth, feed intake, food conversion efficiency and stomach evacuation rate of Atlantic salmon post-smolts[J].Aquaculture,2008,283(1/2/3/4):36-42. [3]BUENTELLO J A, GATLIN D M Ⅲ, NEILL W H. Effects of water temperature and dissolved oxygen on daily feed consumption, feed utilization and growth of channel catfish (Ictalurus punctatus)[J].Aquaculture,2000,182(3/4):339-352. [4]THURSTON R V, PHILLIPS G R, RUSSO R C, et al. Increased toxicity of ammonia to rainbow trout (Salmo gairdneri) resulting from reduced concentrations of dissolved oxygen[J].Canadian Journal of Fisheries and Aquatic Sciences,1981,38(8):983-988. [5]金丹璐,张清科,王友发,等.鲤科经济鱼类马口鱼(Opsariichthys bidens)胚胎发育及仔稚鱼形态与生长观察研究[J].海洋与湖沼,2017,48(4):838-847. [6]陈乘.南方马口鱼的繁殖生物学特性及繁育技术研究[D].长沙:湖南农业大学,2015:1-46. [7]FU S J, PENG Z G, CAO Z D, et al. Habitat-specific locomotor variation among Chinese hook snout carp (Opsariichthys bidens) along a river[J].PLoS One,2012,7(7):e40791. [8]谢建洋.南方马口鱼人工繁育试验报告[J].当代水产,2013,38(10):73. [9]张君.马口鱼的人工繁育技术试验总结[J].河南水产,2015(4):15. [10]练青平,张任驰,宓国强,等.马口鱼规模化人工繁殖技术的初步研究[J].浙江海洋大学学报(自然科学版),2017,36(5):409-413. [11]张胜金戈,段鹏翔,刘小燕,等.南方马口鱼规模化人工繁育技术规范措施分析[J].渔业致富指南,2017(22):59-63. [12]浙江省海洋与渔业局.浙江省淡水所突破马口鱼种苗规模化繁育关键技术[J].渔业致富指南,2017(13):10. [13]TANG D J, GAO X M, LIN C W, et al. Cytological features of spermatogenesis in Opsariichthys bidens (Teleostei, Cyprinidae)[J].Animal Reproduction Science,2020,222:106608. [14]张克鑫,朱卫东,郑春静,等.平原河流水马口鱼苗种繁育技术[J].科学养鱼,2019(12):9-10. [15]郑春静,童鑫良,张克鑫,等.河水土池人工养殖马口鱼试验[J].科学养鱼,2019(10):33-34. [16]张学舒,王英.大黄鱼鱼苗耗氧率和窒息点的研究[J].经济动物学报,2007,11(3):148-152. [17]李欣茹.低氧胁迫对暗纹东方鲀能量代谢、血液指标及基因表达的影响[D].南京:南京师范大学,2018:11-22. [18]杨斯琪,郑洪武,孙颖,等.氨氮、温度和体重对大口黑鲈(Micropterus salmoides)幼鱼耗氧率和窒息点的影响[J].海洋与湖沼,2019,50(6):1328-1333. [19]杨小东,江兴龙,乐普敏,等.南美洲鳗鲡(Anguilla rostrata)的耗氧率(ROC)、窒息点(AP)和适温范围(RT)及对非离子氨(NIA)、NO-2的LC50和SC的研究[J].海洋与湖沼,2019,50(2):455-464. [20]何林强,王万良,曾本和,等.不同规格拉萨裂腹鱼温度耐受性研究[J].水生生物学报,2020,44(6):1230-1238. [21]SOYANO K, MUSHIROBIRA Y. The mechanism of low-temperature tolerance in fish[J].Advances in Experimental Medicine and Biology,2018,1081:149-164. [22]LITTLE A G, LOUGHLAND I, SEEBACHER F. What do warming waters mean for fish physiology and fisheries?[J].Journal of Fish Biology,2020,97(2):328-340. [23]GUDERLEY H. Metabolic responses to low temperature in fish muscle[J].Biological Reviews,2004,79(2):409-427. [24]JOHNSTON I A, DUNN J. Temperature acclimation and metabolism in ectotherms with particular reference to teleost fish[J].Symposia of the Society for Experimental Biology,1987,41:67-93. [25]段娇阳,刘慧,陈四清,等.温度对皱纹盘鲍摄食和耗氧率的影响[J].中国农学通报,2020,36(27):153-157. [26]王丽群,张旭,宿志健,等.体质量和温度对禾花鲤耗氧率与窒息点的影响[J].安徽农业科学,2020,48(10):87-90. [27]徐林通,蔡琰,钟文慧,等.温度对赣昌鲤鲫耗氧率和窒息点影响的研究[J].科学养鱼,2019(9):69-70. [28]何亚,王华,王伟,等.温度对不同体质量红鳍东方鲀幼鱼耗氧率和排氨率的影响[J].大连海洋大学学报,2014,29(5):481-485. [29]付监贵,张振早,李彩娟,等.温度对不同体质量梭鲈幼鱼耗氧率、排氨率及窒息点的影响[J].水产养殖,2018,39(10):18-22. [30]朱世海,闫霜,陈政良.硬刺高原鳅耗氧率与窒息点和体质量关系的研究[J].青海大学学报,2020,38(4):34-38. [31]崔宽宽,尤宏争,丁子元,等.不同规格花鲈的耗氧率与窒息点研究[J].科学养鱼,2018(3):57-58. [32]唐英.南方马口鱼形态、组织和营养特性研究[D].长沙:湖南农业大学,2015:8-18. [33]钟全福,樊海平,叶小军.马口鱼的研究现状及开发利用进展[J].江苏农业科学,2020,48(24):37-41. [34]邱晴,罗文.采用RAPD技术筛选马口鱼性别差异相关分子标记[J].水产养殖,2012,33(1):38-42. [35]宋银都,唐首杰,赵金良.鳜幼鱼窒息点、耗氧率的初步研究[J].水产养殖,2019,40(3):3-5. [36]于燕光,逯云召,薄其康,等.不同温度对大泷六线鱼幼鱼耗氧率和窒息点的影响[J].河北渔业,2020(10):20-23. [37]张善发,董宏标,王茜,等.温度对驼背鲈幼鱼生长、耗氧和热耐受性的影响[J].大连海洋大学学报,2021,36(1):74-79. [38]李育森,雷建军,韩耀全,等.水温和光照强度对乌原鲤耗氧率与临界窒息点的影响[J].南方农业学报,2019,50(2):418-423. [39]王晓光,王兴兵,寇凌霄,等.北方须鳅的耗氧节律、耗氧率和窒息点的研究[J].水产科学,2018,37(3):309-315. [40]杨凯,高银爱,袁勇超,等.赤眼鳟耗氧率、排氨率和窒息点的初步研究[J].淡水渔业,2017,47(5):9-13. [41]徐钢春,聂志娟,薄其康,等.水温对刀鲚幼鱼耗氧率、窒息点、血糖及肌肝糖元指标的影响[J].生态学杂志,2012,31(12):3116-3120. [42]金华.中华胭脂鱼胚胎发育、幼鱼耗氧率及缺氧导致组织病理学的研究[D].天津:天津农学院,2016. [43]冯广朋,庄平,章龙珍,等.温度对中华鲟幼鱼代谢酶和抗氧化酶活性的影响[J].水生生物学报,2012,36(1):137-142. [44]洪磊,张秀梅.环境胁迫对鱼类生理机能的影响[J].海洋科学进展,2004,22(1):114-121. [45]叶林骏,胡艺珂,何昆,等.环境胁迫因子对鱼类氧化压力的影响[J].安徽农业科学,2012,40(29):14326-14330. [46]LUSHCHAK V I, BAGNYUKOVA T V. Hypoxia induces oxidative stress in tissues of a goby, the rotan Perccottus glenii[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2007,148(4):390-397. [47]ZHANG G S, MAO J Q, LIANG F F, et al. Modulated expression and enzymatic activities of darkbarbel catfish, Pelteobagrusvachelli for oxidative stress induced by acute hypoxia and reoxygenation[J].Chemosphere,2016,151:271-279. [48]谭树华,何典翼,严芳,等.亚硝酸钠对鲫鱼肝脏丙二醛含量和总抗氧化能力的影响[J].农业环境科学学报,2005,24(增刊):21-24. [49]蒋玫,李磊,沈新强,等.慢性氨氮胁迫对鲻鱼(Mugil cephalus)幼鱼组织细胞免疫指标的影响研究[J].海洋与湖沼,2014,45(3):529-535.
2023, Vol. 42 
