Analysis of Genetic Diversity of Kuruma Prawn Marsupenaeus japonicus Based on Mitochondrial Cytb and D-Loop Sequences
DU Jinghao1, WANG Weifeng1, CHEN Xiuli2, HOU Chunxiu1, WANG Huanling1
1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China; 2. Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Breeding, Guangxi Zhuang Autonomous Region Academy of Fishery Sciences, Nanning 530021, China
Abstract：About 20 samples of kuruma prawn Marsupenaeus japonicus were collected from 5 coastal waters of Zhoushan, Fuzhou, Xiamen, Beihai and Zhanjiang in Zhejiang Province, Fujian Province, Guangdong Province and Guangxi Zhuang Autonomous Region from August 2018 to March 2019, respectively,and the genetic structure and variation of these five populations were analyzed by mitochondrial cytochrome b gene and DNA control region in muscle of the sixth pleopod to understand the current status of germplasm resources of kuruma prawn in China. Cytb and D-Loop sequences in five populations of kuruma prawn revealed that the A+T content was 60.65% and 82.54%, significantly higher than that of C+G (39.35% and 17.46%). 76 and 75 parsimony informative sites, 55 and 78 of haplotypes were also detected, with haplotype diversity of 0.969 in Cytb and 0.995 in D-Loop, and nucleotide diversity of 0.033 in Cytb and 0.036 in D-Loop. The genetic distance within the populations was varied from 0.004 to 0.022 and 0.005 to 0.017, and the genetic distance among populations was between 0.007 to 0.068 in Cytb and 0.012 to 0.075 in D-Loop. Molecular variance variation analysis showed that inter-population variation was the main source of variation, accounting for 70.01% and 74.39% of the total variation. The haplotype Neighbor-joining tree revealed that Beihai and Zhanjiang populations were clustered together, and the other three populations were clustered into one branch, indicating that the identical system showed different degrees of differentiation. The population demographic analyses revealed that these populations remained relatively stable and had not experienced genetic bottleneck effects and significant expansion. These findings indicated that the five populations of kuruma prawn were more differentiated and had higher genetic diversity, as higher polymorphisms of haplotypes and nucleotides.
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2020, Vol. 39 
