Abstract:To evaluate the genetic diversity of red claw crayfish Cherax quadricarinatus in different breeding farms in China, 180 individuals of six breeding groups (Fuyang in Anhui, Huzhou in Zhejiang, Zhuhai in Guangdong, Zhenjiang in Jiangsu, Chengmai in Hainan, and Suzhou in Jiangsu) were cloned based on mitochondrial cytochrome oxidase subunit Ⅰ gene (COⅠ). Sequence alignment showed that there were 56 variable sites and 16 parsimony-informative sites in total 831 sites. The content of (G+C) was lower (39.765%), showing obvious bias of base composition. Ten haplotypes were detected among the 180 samples. Haplotype H1 found most frequently was shared by the populations from Fuyang, Zhuhai, Zhenjiang, Chengmai and Suzhou. Haplotype diversity index was 0.693 and nucleotide diversity index was 0.00652, indicating that the genetic diversity index of total samples was high. Suzhou population had the highest haplotype diversity index (0.964) while Chengmai population had the lowest haplotype diversity index (0.618) among the six populations. The genetic distance between Chengmai and Fuyang populations was higher than those of others while between Zhuhai and Suzhou is the lowest among the six red claw crayfish groups. The findings indicated that there was high level genetic diversity in red claw crayfish, there were also some gene exchanges among populations. This study has accumulated data for the development and utilization of domestic red claw crayfish germplasm resources.
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