Abstract：In 2018, outbreaks of mass mortalities occurred in giant freshwater prawn Macrobrachium rosenbergii farms in Gaoyou city, Jiangsu Province of China, causing significant economic damage, and Non-O1 Vibrio cholerae is recognized to be the etiological agent of the enzootic. To investigate the immune response, giant freshwater prawn [weight(7.22±0.46)g] was infected withNon-O1 V. cholerae (1×106 cfu/mL) for 24 h. Hepatopancreas tissue with and without non-O1 V. cholerae infection was sampled and then comparative transcriptome analysis was conducted using Illumina Hiseq 2000. After de novo assembly, 85794 high quality unigenes were obtained with an average length of 1208 bp (N50=2804 bp). Searching against the public databases, including GO, COG, KEGG, NR, SWISS and NT, 29731 unigenes were annotated. Differential expression analysis showed that 3236 significantly differently expressed genes (DEGs) were identified with 1745 up-regulated genes and 1419 down-regulated genes. According to the information of gene function annotation, 16 immune-related genes were obtained. The expression level of nine well-known immune-related genes, including LEC 3 and Serpin1, significantly increased. While other seven genes, including HMC and SCARB, showed significantly decreased expression level. The dominant enriched GO terms of DEGs were involved in cellular process, cell and binding. Three GO terms, including immune system process, response to stimulus, and antioxidant activity, involved in immune response, were enriched. This result demonstrated that non-O1 V. cholerae infection stimulated and activate the antioxidant system and further triggers non-self-recognition molecules and immune response. KEGG analysis revealed that DEGs were enriched into 121 pathways including several immune-related pathways, such as phagosome, ECM-receptor interaction, Vibrio cholerae infection, leukocyte transendothelial migration, NOD-like receptor signaling pathway and so on. To verify the accuracy of transcriptome sequencing, eight DEGs were selected to conduct quantitative real-time PCR. Results of qRT-PCR were consistent with RNA-seq, which indicated that the differential gene expression results based on the transcriptome sequencing were credible. Data from this study provided part of basic information about gene expression changes of hepatopancreas after non-O1 V. cholerae infection, which might be helpful for further studying the immune response of giant freshwater prawn against non-O1 V. cholerae infection.
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2023, Vol. 42 
