Isolation, Identification and Antibiotic Sensitivity of Pathogen Stenotrophomonas maltophilia from Hybrid Sturgeon
SHANG Baodi1,2, TAO Sha3, YANG Xing1,2, LI Xiaoyi1.2, LI Zhengyou1,2, ZHANG Xiaoping1,2, KONG Jie1,2
1. Guizhou Fisheries Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China; 2. Guizhou Special Aquatic Products Engineering Technology Center, Guiyang 550025, China; 3. Guizhou University, Guiyang 550025, China
Abstract：Morphological observation, physiological and biochemical characteristics, 16S rDNA and gyrB gene sequence analysis were used to identify a suspected pathogen strain SXG-1 isolated and purified from the liver of the diseased sturgeon hybrid [kaluga sturgeon (Huso dauricus)♀×Amur sturgeon(Acipenser schrenckii)♂] collected from a farm in Suiyang district, Guizhou Province, in September to October of 2017, with cumulative mortality of 20% in order to effectively promote the development of sturgeon breeding industry in Guizhou Province and to reduce the damage caused by disease outbreaks. The strain SXG-1 was identified as Stenotrophomonas maltophilia. Artificial infected fish by intraperitoneal SXG-1-injection showed same symptoms as that of natural ones with similar morphological and biochemical characteristics, proving to be the pathogen of the hybrid sturgeon, with LD50 of 3.9×108 cfu/mL. The antibiotic sensitivity test revealed that the isolate was sensitive to enrofloxacin and florfenicol. The finding provides scientific guidance to the prevention and control the disease of sturgeon.
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2020, Vol. 39 
