Polymorphism Analysis of Mitochondria Cyt b Gene Sequences in Wild Population of LiBo Spot Mandarin Fish Siniperca scherzeri
MA Zhifang1, YU Ke1, AN Miao1, HUANG Sheng1, PAN Qiuzhi1, LI Shan2, HE Xiaokuan3
1. College of Animal Science, Guizhou University, Guiyang 550025, China; 2. Guizhou Vocational College of Agriculture, Guiyang 551499, China; 3. Agriculture and Rural Affairs Bureau of Libo County, Qiannan 558400, China
Abstract：In order to understand the status of germplasm resources and the level of genetic variation of wild population of LiBo spot mandarin fish Siniperca scherzeri, the cytochrome b (Cyt b) gene marker was selected for the genetic diversity analysis. The completed mt DNA Cytb gene sequence(1141 bp) of wild spot mandarin fish in Zhangjiang Libo County, Guizhou Province, was obtained by PCR amplification and sequence analysis. The amplification showed that the average contents of A,T,G and C were 24.4%, 27.2%, 15.5% and 32.9%, respectively, a little higher average content of A+T(51.6%) than that of G+C (48.4%). Seventeen variation sites were detected, in these variation sites where eight were parsimony informative sites and nine were singleton variable sites. Fifteen haplotypes were identified in 75 individuals of the population, with the average genetic distance of 0.003 among Cyt b haplotypes, and the phylogenetic tree revealed that fifteen Cyt b haplotypes were clustered into one clade, with haplotype diversity of 0.613±0.064 and nucleotide diversity of 0.00172±0.00021, and the average number of nucleotide differences being 1.986. The Tajima's D value of neutrality test was －1.37917, without significant difference (P＞0.10). These findings indicated that the wild population of LiBo spot mandarin fish had low genetic diversity, as high haplotype diversity and low nucleotide diversity. The population demographic analyses revealed that the wild population of LiBo spot mandarin fish had a stable population without experiencing population expansion, with the closest evolutionary relationship with the spot mandarin fish from Yangtze River (Lushui and Dongting)according to a phylogenetic study. The finding provides reference for the protection and utilization of its germplasm resources.
麻智芳, 余科, 安苗, 黄胜, 潘秋芝, 李珊, 何孝宽. 荔波樟江野生斑鳜线粒体Cyt b基因序列多态性分析[J]. 水产科学, 2023, 42(1): 21-29.
MA Zhifang, YU Ke, AN Miao, HUANG Sheng, PAN Qiuzhi, LI Shan, HE Xiaokuan. Polymorphism Analysis of Mitochondria Cyt b Gene Sequences in Wild Population of LiBo Spot Mandarin Fish Siniperca scherzeri. Fisheries Science, 2023, 42(1): 21-29.
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2023, Vol. 42 
