Abstract：Amur sleeper Perccottus glenii with body weight of (13.41±6.31) g was divided into three groups and reared in a glass aquarium of 80 cm×60 cm×40 cm at water temperature of 17 ℃ (control group), gradual warming at a rate of 1 ℃/d (gradual warming group), and in acute warming group, the test fish being transferred directly from 17 ℃ aquarium to 31 ℃ aquarium. In the two groups the water temperature was kept at constant 31 ℃ and the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) and malondialdehyde (MDA) content were determined in liver, brain and muscle of Amur sleeper to understand the effects of acute warming stress on the antioxidant capacity and mortality rate of Amur sleeper and to provide references with industrial aquaculture of Amur sleeper. The results showed that there were significantly higher mortality rate and activities of antioxidant enzymes in liver and brain in the Amur sleeper in the acute warming group than those in the gradual warming group (P<0.05). The activities of SOD and GPX in brain were found to be significantly higher in the Amur sleeper in the acute warming group than those in gradually warming group after 6 hours (P<0.05). The activities of SOD and GPX, however, were significantly lower than those in the control group during the recovery to 17 ℃ (P<0.05), and the activities of SOD in muscle were significantly higher than those in gradually warming group at 12 hours (P<0.05). The content of MDA in liver was significantly higher in the Amur sleeper in the acute warming group than that in control group within 1 hour (P<0.05). The content of MDA in the brain was significantly higher than that in the control group and the gradually warming group (P<0.05), and in the muscle was significantly higher than that in the gradually warming group (P<0.05), while the content of MDA in the brain during recovery was significantly higher than that in the control group (P<0.05). The findings indicated that liver and brain injury was not recovered under long-term high temperature stress, and muscle was more adaptable to acute warming than liver and brain. GPX in the brain first responded to oxidative damage caused by acute warming, and then increases in SOD and CAT activities inhibitting GPX activity. Amur sleeper can survive longer under the condition of gradual warming than under acute warming.
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2020, Vol. 39 
