1. Key Laboratory of Animal Genetics and Breeding and Reproduction of Plateau and Mountain Animals, College of Animal Science, Guizhou University, Guiyang 550025, China; 2. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. Guizhou Institute of Socialism, Guiyang 550005,China; 4. Guizhou Fisheries Reasarch Institute, Guiyang 550025, China
Abstract：In order to investigate the changes in morphological character and autioxidant enzyme activities during the embryo development of giant spiny frog Quasipaa spinosa, the cultured breeding parents were prepared to mature and spawn in imitation ecology condition. The whole process of embryonic development was continuously observed, photographed, and measured. The activities of autioxidant enzymes were identified follow the instructions. The fertilized egg was belong to the sinuous viscous egg with (4.31±0.69) mm in length. The results showed that under an incubation temperature of (24.5±0.5) ℃, it took 222.94 h for the whole embryonic development to hatching stage and 288.44 h to yolk exhausted stage with the total accumulative temperature of 470.95 h·℃ and 7078.32 h·℃, respectively. According to the external morphology and typical characteristics of embryonic development, it can be divided into seven stages. The activities of superoxide dismutase, catalase and glutamic-pyruvic transaminase showed the same trend as well as the malondialdehyde content significantly decreased in the mid-gastrula stage. The activity of glutamic oxalacetic transaminase was reached a peak in the same time, then showed continuous declination. In addition, the activities of catalase, superoxide dismutase glutamic-pyruvic transaminase and glutamic-pyruvic transaminase were minimal in the yolk absorbs stage, while the lowest levels of malondialdehyde was observed in the mid-gastrula stage. The finding will provide basic data and important references for seed production technology efficiently in the future.
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2023, Vol. 42 
