Metabolism and Hepatotoxicity of Mebendazole in Allogenic Silver Crucian Carp
SU Meizhen1, ZHANG Yitong2, WANG Hao3, LYU Liqun1
1. National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, China; 2. Key Laboratory of Freshwater Aquatic Genetic Resources for Aquaculture, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; 3. National Experimental Teaching Demonstration Center for Fishery Sciences, Shanghai Ocean University, Shanghai 201306, China
Abstract：Allogenic silver crucian carp Carassius auratus gibelio with body weight of (90±20) g was intragastrically administered with mebendazole at a dose of 20 mg/kg, 10 mg/kg or 5 mg/kg, while the control group was orally administered with formic acid,at water temperature of (20±2) ℃ and blood was collected from caudal vein at 1, 2, 4, 6, 10, 24, 48 and 84 hours after administration. Potassium oxalate anticoagulant (anticoagulant∶plasma=1∶10,V/V) was added immediately into the blood samples which then were centrifugated at 4000 rpm for 10 min. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione catalase (GSH-PX), glutathione transaminase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (AKP) and the transcriptional expression levels of SOD and CAT genes were compared in the hepatopancreas and kidney of allogenic silver crucian carp at 0, 24 and 48 hours after administration. It was found that there were similar changes in the three tissues after administration, with the maximal content of mebendazole in three tissues of crucian carp in 24 hours, and then gradually metabolized, with the maximum in hepatopancreas, followed by kidney, and the minimum in blood. The maximal concentration of mebendazole in hepatopancreas was about 1.3 times as that in kidney and 45 times as that in blood under administration at 20 mg/kg. The contents of glutathione catalase in liver and pancreas of tobendazole-treated fish were decreased in a time-dependent manner, while the activities of alkaline phosphatase, catalase, superoxide dismutase, glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase in blood were increased first and then decreased. The findings indicated that oral administration of 20 mg/kg mebendazole had a negative impact on various indicators, damaging normal detoxification and antioxidant function, which provides theoretical support for the safe use of mebendazole in aquaculture.
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2020, Vol. 39 
