Effects of Acute Ammonia Stress on Tissue Structure of Main Organs of Chinese Striped-Necked Turtle Mauremys sinensis
HUANG Zubin, LIANG Lingyue, LI Weihao, LI Na, DING Li, HONG Meiling
Key Laboratory of Tropical Island Ecology, Ministry of Education, Hainan Key Laboratory of Tropical Animal and Plant Ecology, College of Life Sciences, Hainan Normal University, Haikou 571158, China
Abstract：In order to explore the effects of acute ammonia nitrogen stress on the tissue structure of main organs, one-year-old Chinese striped-necked turtle Mauremys sinensis with average body weight of (218.26±12.65) g was treated with 200 mg/L total ammonia nitrogen for 24 h, 48 h and removal of ammonia nitrogen for 48 h. The effects of ammonia nitrogen stress on the tissue structure of brain, liver, heart, spleen and kidney were studied by H·E staining.The results showed that there were broadening and loosening interstitium, deviation of neuronal nucleus, degeneration and necrosis of neurons after ammonia nitrogen stress, and the phenomenon of neuronal swelling and nuclear deviation was found to be decreased after 48 hours of recovery. Pathological phenomena such as hepatocyte swelling, granular degeneration, vesicular degeneration and necrosis were found in the liver, and the hepatic sinusoid shrunk or even disappeared. After 48 hours of recovery, the hepatocyte swelling was decreased and the hepatic sinusoid basically returned to the pre-stress level. However, the phenomena of hepatocyte granule degeneration and vesicular degeneration still existed; cardiac interstitial vascular congestion, the myocardial intercellular space shrinked to the pre-stress level 48 hours after recovery, and the phenomenon of hyperemia disappeared. Renal tubular epithelial cells had granular degeneration, some cells fell off from the basement membrane, cell membrane ruptured, glomerular volume increased, capillary network dilated, nuclear pyknosis or even dissolution disappeared, and inflammatory cells infiltrated in the interstitium. Inflammatory cells basically disappeared after 48 hours of recovery, but pathological phenomena such as irregular lumen of renal tubules and granular degeneration still existed. The white pulp area of the spleen enlarged, and the white marrow and red marrow tended to the normal level after 48 hours of recovery. It was found that acute ammonia nitrogen stress caused varying degrees of damage to the brain, liver, heart and kidney of Chinese striped-necked turtles, and 48 hours of recovery was not enough for Chinese striped-necked turtle to fully recover under stress, in which the recovery ability of heart was better, the recovery ability of brain, liver and kidney was relatively poor, and the proliferation of immune cells in spleen indicated that the body initiated immune defense mechanism after stress to enhance the immune ability of the body.The findings expounded the effect of ammonia nitrogen stress on Chinese striped-necked turtles from the point of view of pathology, which provided a theoretical basis for the healthy culture of turtles.
黄祖彬, 梁龄月, 李维昊, 李娜, 丁利, 洪美玲. 急性氨氮胁迫和恢复后中华条颈龟主要器官组织结构的变化[J]. 水产科学, 2022, 41(1): 143-149.
HUANG Zubin, LIANG Lingyue, LI Weihao, LI Na, DING Li, HONG Meiling. Effects of Acute Ammonia Stress on Tissue Structure of Main Organs of Chinese Striped-Necked Turtle Mauremys sinensis. Fisheries Science, 2022, 41(1): 143-149.
[1]裘正元,盛成,洪美玲,等.豆油替代鱼油对中华条颈龟血脂代谢及相关酶活的影响[J].水产科学,2015,34(7):453-458. [2]赵春光.我国本土名龟的养殖现状与前景展望[J].中国水产,2018(4):30-33. [3]REN H, LI J, LI J T, et al. Transcript profiles of mitochondrial and cytoplasmic manganese superoxide dismutases in Exopalaemon carinicauda under ammonia stress[J].Chinese Journal of Oceanology and Limnology,2015,33(3):714-724. [4]CHEN Y Y, SIM S S, CHIEW S L, et al. Dietary administration of a Gracilaria tenuistipitata extract produces protective immunity of white shrimp Litopenaeus vannamei in response to ammonia stress[J].Aquaculture,2012,370/371:26-31. [5]LIANG Z X, LIU R, ZHAO D P, et al. Ammonia exposure induces oxidative stress, endoplasmic reticulum stress and apoptosis in hepatopancreas of Pacific white shrimp (Litopenaeus vannamei)[J].Fish & Shellfish Immunology,2016,54:523-528. [6]BENLI A Ç K, KÖKSAL G, ÖZKUL A. Sublethal ammonia exposure of Nile tilapia (Oreochromis niloticus L.):effects on gill, liver and kidney histology[J].Chemosphere,2008,72(9):1355-1358. [7]RUYET P L, LAMERS A, ROUX A L, et al. Long-term ammonia exposure of turbot:effects on plasma parameters[J].Journal of Fish Biology,2010,62(4):879-894. [8]PAUST L O, FOSS A, IMSLAND A K. Effects of chronic and periodic exposure to ammonia on growth, food conversion efficiency and blood physiology in juvenile Atlantic halibut (Hippoglossus hippoglossus L.)[J].Aquaculture,2011,315(3/4):400-406. [9]陈思涵,彭瑞冰,黄晨,等.急性氨氮胁迫对虎斑乌贼肝脏、鳃和脑组织结构的影响[J].水产学报,2018,42(9):1348-1357. [10]张武肖,孙盛明,戈贤平,等.急性氨氮胁迫及毒后恢复对团头鲂幼鱼鳃、肝和肾组织结构的影响[J].水产学报,2015,39(2):233-244. [11]许星鸿,张雁秋,阎斌伦,等.氨氮胁迫对日本蟳免疫生理指标及器官结构的影响[J].生态学报,2014,34(14):3885-3894. [12]吴利敏,徐瑜凤,李永婧,等.急性氨氮胁迫对淇河鲫幼鱼脑、鳃、肝、肾组织结构的影响[J].中国水产科学,2020,27(7):789-800. [13]洪美玲,陈立侨,顾顺樟,等.氨氮胁迫对中华绒螯蟹免疫指标及肝胰腺组织结构的影响[J].中国水产科学,2007,14(3):412-418. [14]王贞杰,陈四清,曹栋正,等.急性氨氮胁迫对圆斑星鲽(Verasper variegatus)幼鱼鳃和肝组织结构及相关酶活性的影响[J].渔业科学进展,2017,38(2):59-69. [15]郝小凤,刘洋,凌去非.氨氮对泥鳅的急性毒性及对其肝、鳃组织超微结构的影响[J].水生态学杂志,2012,33(5):101-107. [16]严福祥,李贵生,付晓艳.中华花龟五种器官组织学观察[J].生态科学,2010,29(1):75-79. [17]雷雯雯.镉对长江华溪蟹心脏组织器官的毒性作用及机制研究[D].太原:山西大学,2012. [18]王来,姚素梅,王强.镉的心脏毒性[J].环境与职业医学,2006,23(5):436-439. [19]MISHRA A K, MOHANTY B. Acute toxicity impacts of hexavalent chromium on behavior and histopathology of gill, kidney and liver of the freshwater fish, Channa punctatus (Bloch)[J].Environmental Toxicology and Pharmacology,2008,26(2):136-141. [20]王晓杰,张秀梅,姜明.盐度胁迫对许氏平鮋鳃、头肾、脾脏超微结构的影响[J].中国海洋大学学报(自然科学版),2006,36(增刊):85-90. [21]王文博,李爱华,汪建国,等.拥挤胁迫对草鱼非特异性免疫功能的影响[J].水产学报,2004,28(2):139-144. [22]刘奇奇,温久福,区又君,等.运输胁迫对四指马鲅幼鱼肝脏、鳃和脾脏组织结构的影响[J].南方农业学报,2017,48(9):1708-1714.
2022, Vol. 41 
