Effects of Acute Hypoxia Stress on Gill Structure, Physiology and Biochemistry of Hybrid F3 of Blunt Snout Bream Megalobrama amblycephala ♀× Culter Culter alburnus ♂
CHEN Songlin, LIN Xin, ZHENG Guodong, ZOU Shuming
Genetics and Breeding Center for Blunt Snout Bream, Ministry of Agriculture and Rural Affairs, Key Laboratory ofFreshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, National Demonstration Centerfor Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract：By adjusting the amount of nitrogen or air in the water, hybrid F3 of blunt snout bream Megalobrama amblycephala (♀) × culter Culter alburnus (♂) was exposed to normal [dissolved oxygen concentration = (7.16±0.20) mg/L], low oxygen [dissolved oxygen concentration = (1.04±0.12) mg/L] and recover [dissolved oxygen concentration = (7.19±0.23) mg/L]. Gills of hybrid F3 of normal, hypoxia 12 h, hypoxia 24 h, hypoxia 72 h and recovery were used as samples. Hematoxylin-Eosin staining, Alcian blue-Periodie acid Schiff staining, antioxidant enzyme activity determination, qRT-PCR and other techniques were used to explore the adaptability of gill tissue of hybrid F3 to hypoxia. The results showed that after 72 h of hypoxic treatment, the area of intercellular cell mass in gill tissue of hybrid F3 was significantly reduced, the length of gill lamellae was prolonged, and the contact area between gill lamellae and water was increased, so as to adapt to hypoxic environment. The area of interlamellar cell mass was recovered after reoxygenation. Hypoxic stress led to significantly reduce the density of mucus cells in gill tissue of hybrid F3, and reduce the secretion function of mucus in gill tissue. Under hypoxia stress, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in gill tissue of hybrid F3 were the maximum at 72 h, and catalase (CAT) activity was the maximum at 24 h. Hypoxia stress significantly changed the mRNA expression levels of caspase-3 and Bcl-2 genes in gill tissue, with the different trends. In the 72 h hypoxia treatment group, the expression level of caspase-3 gene was the maximum and that of Bcl-2 gene was the minimum. After 24 h of reoxygenation treatment, the structure, physiological and biochemical indices of gill tissue was recovered. In summary, hypoxia environment significantly affected the morphological structure, mucus secretion function, and antioxidant enzyme activity of gill tissue of hybrid F3, but did not completely destroy its self-regulation ability.
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2023, Vol. 42 
